Improved guide RNA and Protein Design for CasX-based Gene Editing Platform
Patent Status
| Country | Type | Number | Dated | Case |
| United States Of America | Published Application | 20230407276 | 12/21/2023 | 2021-064 |
| European Patent Office | Published Application | 4256045 A0 | 10/11/2023 | 2021-064 |
Brief Description
The inventors have developed two new CasX gene-editing platforms (DpbCasXv2 and PlmCasXv2) through rationale structural engineering of the CasX protein and gRNA, which yield improved in vitro and in vivo behaviors. These platforms dramatically increase DNA cleavage activity and can be used as the basis for further improving CasX tools.
The RNA-guided CRISPR-associated (Cas) protein CasX has been reported as a fundamentally distinct, RNA-guided platform compared to Cas9 and Cpf1. Structural studies revealed structural differences within the nucleotide-binding loops of CasX, with a compact protein size less than 1,000 amino acids, and guide RNA (gRNA) scaffold stem. These structural differences affect the active ternary complex assembly, leading to different in vivo and in vitro behaviors of these two enzymes.
Suggested uses
Advantages
Improved in vitro and in vivo behaviors of CasX and guide RNA.
Related Materials
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Additional Technologies by these Inventors
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- NANOPORE MEMBRANE DEVICE AND METHODS OF USE THEREOF
- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
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- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
- Engineered/Variant Hyperactive CRISPR CasPhi Enzymes And Methods Of Use Thereof
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- Type V CRISPR/CAS Effector Proteins for Cleaving ssDNA and Detecting Target DNA
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- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Endoribonucleases For Rna Detection And Analysis
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- CRISPR-Cas Effector Polypeptides and Methods of Use Thereof
- Class 2 CRISPR/Cas COMPOSITIONS AND METHODS OF USE
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
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- Methods and Compositions for Controlling Gene Expression by RNA Processing
