Single Conjugative Vector for Genome Editing by RNA-guided Transposition
Tech ID: 31691 / UC Case 2020-057-0
The inventors have constructed conjugative plasmids for intra- and inter-species delivery and expression of RNA-guided CRISPR-Cas transposases for organism- and site-specific genome editing by targeted transposon insertion. This invention enables integration of large, customizable DNA segments (encoded within a transposon) into prokaryotic genomes at specific locations and with low rates of off-target integration.
Microbial strain development for heterologous expression of large DNA segments; integrating large segments of DNA; integrating biosynthetic gene clusters; integrating polysaccharide utilization loci; genome minimization; genome reorganization; genome editing in microbial communities; genome editing in microbial isolates; genome editing in microbes that are recalcitrant to plasmid transformation by heat shock or electroporation; genome editing in strains in which homologous recombination or other repair-based editing is not feasible.