Single Conjugative Vector for Genome Editing by RNA-guided Transposition
Tech ID: 31691 / UC Case 2020-057-0
Patent Status
| Country | Type | Number | Dated | Case |
| United States Of America | Published Application | 20230068726 | 03/02/2023 | 2020-057 |
| European Patent Office | Published Application | 4097225 A0 | 12/07/2022 | 2020-057 |
Brief Description
The inventors have constructed conjugative plasmids for intra- and inter-species delivery and expression of RNA-guided CRISPR-Cas transposases for organism- and site-specific genome editing by targeted transposon insertion. This invention enables integration of large, customizable DNA segments (encoded within a transposon) into prokaryotic genomes at specific locations and with low rates of off-target integration.
Suggested uses
Microbial strain development for heterologous expression of large DNA segments; integrating large segments of DNA; integrating biosynthetic gene clusters; integrating polysaccharide utilization loci; genome minimization; genome reorganization; genome editing in microbial communities; genome editing in microbial isolates; genome editing in microbes that are recalcitrant to plasmid transformation by heat shock or electroporation; genome editing in strains in which homologous recombination or other repair-based editing is not feasible.
Advantages
Related Materials
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Keywords
CRISPR-Cas, conjugative plasmids, genome editing, transposon
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- CasX Nickase Designs, Tans Cleavage Designs & Structure
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- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
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- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
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- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
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