Single Conjugative Vector for Genome Editing by RNA-guided Transposition

Tech ID: 31691 / UC Case 2020-057-0

Patent Status

Patent Pending

Brief Description

The inventors have constructed conjugative plasmids for intra- and inter-species delivery and expression of RNA-guided CRISPR-Cas transposases for organism- and site-specific genome editing by targeted transposon insertion. This invention enables integration of large, customizable DNA segments (encoded within a transposon) into prokaryotic genomes at specific locations and with low rates of off-target integration.

Suggested uses

Microbial strain development for heterologous expression of large DNA segments; integrating large segments of DNA; integrating biosynthetic gene clusters; integrating polysaccharide utilization loci; genome minimization; genome reorganization; genome editing in microbial communities; genome editing in microbial isolates; genome editing in microbes that are recalcitrant to plasmid transformation by heat shock or electroporation; genome editing in strains in which homologous recombination or other repair-based editing is not feasible.

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

CRISPR-Cas, conjugative plasmids, genome editing, transposon

Categorized As

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