Efficient Site-Specific Integration Of New Genetic Information Into Human Cells

Tech ID: 24215 / UC Case 2014-199-0

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,570,418 02/25/2020 2014-199

Brief Description


The CRISPR/Cas9 system is a robust genome editing technology that works based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme.  Cas9 generates blunt double-strand DNA (dsDNA) breaks (DSBs) at sites defined by a guide sequence contained within an associated CRISPR RNA (crRNA) transcript and has transformed the ability to engineer eukaryotic organisms by initiating DNA repair pathways that lead to targeted genetic re-programming.  There is a need, however, for methods that increase the efficiency of target DNA modification by Cas9 targeting complexes and/or increase the effectiveness of such methods.


UC Berkeley researchers have found a simple and robust approach for introducing new genetic information site-specifically, in a eukaryotic cell, and with high efficiency using timed delivery of Cas9-guide RNA ribonucleoprotein (RNP) complexes.  The approach combines well-established cell cycle synchronization techniques with direct nucleofection of the pre-assembled Cas9 ribonucleoprotein (RNP) complexes to achieve controlled nuclease action at the phase of the cell cycle best for HDR.


Suggested uses

  • Genome editing (e.g., human, plants, and animal cells)
  • Genome engineering in both transformed and primary human cells


  • Enhanced HDR efficiency in human cells
  • Highly efficient, site-specific editing
  • Simple and robust editing
  • Cell mortality minimized


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  • Doudna, Jennifer A.

Other Information


Genetic engineering, homology-directed repair, HDR, genome editing, Cas9, CRISPR

Categorized As

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