RNA-directed Cleavage and Modification of DNA using CasY (CRISPR-CasY)

Tech ID: 26043 / UC Case 2017-017-0

Patent Status

Country Type Number Dated Case
Eurasian Patent Office Issued Patent 045278 11/10/2023 2017-017
Japan Issued Patent 7306696 07/03/2023 2017-017
United Kingdom Issued Patent 2569734 09/07/2022 2017-017
United States Of America Issued Patent 11,371,062 06/28/2022 2017-017
United States Of America Published Application 20220396812 12/15/2022 2017-017
Mexico Published Application WO 2018/064352 09/25/2020 2017-017
Hong Kong Published Application 40014082 08/14/2020 2017-017
Hong Kong Published Application 40013668A 08/07/2020 2017-017
European Patent Office Published Application 3532089 A0 09/04/2019 2017-017
India Published Application 28/2019 07/12/2019 2017-017
Brazil Published Application 2529 06/25/2019 2017-017
Australia Published Application WO 2018/064352 04/05/2018 2017-017
Canada Published Application WO 2018/064352 04/05/2018 2017-017
China Published Application WO 2018/064352 04/05/2018 2017-017
Israel Published Application WO 2018/064352 04/05/2018 2017-017
Rep Of Korea Published Application WO 2018/064352 04/05/2018 2017-017
New Zealand Published Application WO 2018/064352 04/05/2018 2017-017
Saudi Arabia Published Application WO 2018/064352 04/05/2018 2017-017
Singapore Published Application WO 2018/064352 04/05/2018 2017-017
South Africa Published Application WO 2018/064352 04/05/2018 2017-017
 

Additional Patent Pending

Brief Description


The CRISPR-Cas system is now understood to confer bacteria and archaea with acquired immunity against phage and viruses. CRISPR-Cas systems consist of Cas proteins, which are involved in acquisition, targeting and cleavage of foreign DNA or RNA, and a CRISPR array, which includes direct repeats flanking short spacer sequences that guide Cas proteins to their targets.  Class 2 CRISPR-Cas are streamlined versions in which a single Cas protein bound to RNA is responsible for binding to and cleavage of a targeted sequence. The programmable nature of these minimal systems has facilitated their use as a versatile technology that is revolutionizing the field of genome manipulation.  Current CRISPR Cas technologies are based on systems from cultured bacteria, leaving untapped the vast majority of organisms that have not been isolated.  There is a need in the art for additional Class 2 CRISPR/Cas systems (e.g., Cas protein plus guide RNA combinations).

 

 

UC Berkeley researchers discovered a new type of Cas protein, CasY.  CasY is short compared to previously identified CRISPR-Cas endonucleases, and thus use of this protein as an alternative provides the advantage that the nucleotide sequence encoding the protein is relatively short.  CasY utilizes a guide RNA to perform double stranded cleavage of DNA. The researchers introduced CRISPR-CasY into E. coli, finding that they could block genetic material introduced into the cell.  Further research results indicated that CRISPR-CasY operates in a manner analogous to CRISPR-Cas9, but utilizing an entirely distinct protein architecture containing different catalytic domains.   CasY is also expected to function under different conditions (e.g., temperature) given the environment of the organisms that CasY was expressed in.  Similar to CRISPR Cas9, CasY enzymes are expected to have a wide variety of applications in genome editing and nucleic acid manipulation. 

 

Suggested uses


  • Diagnostics

 

Advantages


  • Functions under different conditions than current CRISPR-Cas proteins (e.g., lower temperatures)
  • Nucleotide sequence encoding the CasY protein is short

Publication

New CRISPR–Cas systems from uncultivated microbes 

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

CRISPR, gene editing, genome, gene therapy, cell biology, CasY, Cas12d

Categorized As

Additional Technologies by these Inventors