Imaging of the eye is routine in the diagnosis of glaucoma, but optic imaging is increasingly playing a role in the diagnosis and monitoring of neurodegenerative diseases, such as retina disease, multiple sclerosis (MS), Alzheimer’s disease, and Parkinson’s disease. Non-invasive imaging of the eye in a quantitative manner is now possible using OCT. Optical coherence tomography (OCT) imaging allows the visualization of biological tissues at high resolution, combined with ease of use, patient comfort, and low cost. This technology allows the discrimination of healthy from diseased tissue, and for the diagnosing and monitoring of disease. These benefits have made OCT the instrument of choice for imaging retinal cell layers in both ophthalmology and neurology imaging, including for monitoring neurodegenerative disease. Unfortunately, following imaging, manual segmentation of images of the optic nerve head (ONH) structures is a time-consuming process that is impractical for routine clinical use. A pressing challenge is to be able to quickly, effectively, and accurately, image the multiple layers of cells within the retina.