Near infrared (NIR) fluorescence imaging (FI) utilizing the fluorophore indocyanine green (ICG) has become more popular for use in medical diagnostics. It is useful for assessing tissue perfusion in a number of surgeries, particularly abdominal, heart, plastic, hepatic as well as other areas of medicine. The light needed for the excitation of the fluorescence is generated by a near infrared light source which is attached directly to a camera. A digital video camera allows the absorption of the ICG fluorescence to be recorded in real time, which means that perfusion can be assessed and documented. Currently, ICG provides a visual representation of tissue perfusion as a global view. Although some efforts have been put into density analysis, no device or software currently performs dynamic evaluation of blood flow for a surgeon. Without objective dynamic measurements, practitioners are only limited to snap shot view of the static environment. This is a problem because it is the dynamics of blood flow that determines tissue perfusion, not how much blood present at a stationary point in time. Furthermore, because there are no numerical evaluations out on the market that can capture this dynamic aspect of blood flow, practitioners are forced to use the naked eye to make a clinical decision that is not only subjective, but is difficult to assess between cases.