Single Conjugative Vector for Genome Editing by RNA-guided Transposition
Tech ID: 31691 / UC Case 2020-057-0
Patent Status
| Country | Type | Number | Dated | Case |
| United States Of America | Published Application | 20230068726 | 03/02/2023 | 2020-057 |
Brief Description
The inventors have constructed conjugative plasmids for intra- and inter-species delivery and expression of RNA-guided CRISPR-Cas transposases for organism- and site-specific genome editing by targeted transposon insertion. This invention enables integration of large, customizable DNA segments (encoded within a transposon) into prokaryotic genomes at specific locations and with low rates of off-target integration.
Suggested uses
Microbial strain development for heterologous expression of large DNA segments; integrating large segments of DNA; integrating biosynthetic gene clusters; integrating polysaccharide utilization loci; genome minimization; genome reorganization; genome editing in microbial communities; genome editing in microbial isolates; genome editing in microbes that are recalcitrant to plasmid transformation by heat shock or electroporation; genome editing in strains in which homologous recombination or other repair-based editing is not feasible.
Advantages
Related Materials
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Keywords
CRISPR-Cas, conjugative plasmids, genome editing, transposon
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- Cas12-mediated DNA Detection Reporter Molecules
- Improved guide RNA and Protein Design for CasX-based Gene Editing Platform
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
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- RNA-directed Cleavage and Modification of DNA using CasY (CRISPR-CasY)
- CasX Nickase Designs, Tans Cleavage Designs & Structure
- In Vivo Gene Editing Of Tau Locus Via Liponanoparticle Delivery
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- A Protein Inhibitor Of Cas9
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- IS110 and IS1111 Family RNA-Guided Transposons
- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
- Variant Cas12a Protein Compositions and Methods of Use
- In Vitro and In Vivo Genome Editing by LNP Delivery of CRISPR Ribonucleoprotein
- CRISPR CASY COMPOSITIONS AND METHODS OF USE
- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
- Engineered/Variant Hyperactive CRISPR CasPhi Enzymes And Methods Of Use Thereof
- Methods Of Use Of Cas12L/CasLambda In Plants
- Type V CRISPR/CAS Effector Proteins for Cleaving ssDNA and Detecting Target DNA
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Variant TnpB and wRNA Proteins
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
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- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
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