Variant Cas12a Protein Compositions and Methods of Use
Patent Status
Patent Pending
Brief Description
Class 2 CRISPR-Cas are streamlined versions in which a single Cas protein bound to RNA is responsible for binding to and cleavage of a targeted sequence. The
programmable nature of these minimal systems has facilitated their use as a versatile technology for genome editing. CRISPR-Cas enzymes with reduced requirements for a protospacer-adjacent motif (PAM) sequence adjacent to the target site could improve the breadth of target sites available for genome editing.
UC Berkeley researchers have developed a novel PAM-loose 12a variants, nucleic acids encoding the variant Cas12a proteins and systems using these variants that make the Cas12a-based CRISPR technology much easier to design a DNA target for carrying out genome editing in human cells.
Suggested uses
- Gene editing
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Additional Technologies by these Inventors
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- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
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- Methods Of Use Of Cas12L/CasLambda In Plants
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- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- Virus-encoded DNA-binding Proteins
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- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
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