Cas12-mediated DNA Detection Reporter Molecules

Tech ID: 29426 / UC Case 2018-173-0

Patent Status

Patent Pending

Brief Description

Class 2 CRISPR-Cas systems are streamlined versions in which a single Cas protein (an effector protein, e.g., a type V Cas effector protein such as Cpf1) bound to RNA is responsible for binding to and cleavage of a targeted sequence. The programmable nature of these minimal systems has facilitated their use as a versatile technology that continues to revolutionize the field of genome manipulation. 

 

Cas12 is an RNA-guided protein that binds and cuts any matching DNA sequence. Binding of the Cas12-CRISPR RNA (crRNA) complex to a matching single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) molecule activates the protein to non-specifically degrade any ssDNA in trans. Cas12a-dependent target binding can be coupled to a reporter molecule to provide a direct readout for DNA detection within a sample.  UC Berkeley researchers have developed compositions, systems, and kits having labeled single stranded reporter DNA molecules that provide a sensitive readout for detection of a target DNA. 

Suggested uses


  • detecting a target DNA (double stranded or single stranded) in a sample

Advantages


  • increased speed and sensitivity of nucleic acid detection

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

Detector, reporter, CRISPR, Cas12

Categorized As