Miniature Type VI CRISPR-Cas Systems and Methods of Use
Tech ID: 32413 / UC Case 2021-183-0
Patent Status
| Country | Type | Number | Dated | Case |
| Patent Cooperation Treaty | Reference for National Filings | WO 2023/201203 | 10/19/2023 | 2021-183 |
Patent Pending
Brief Description
This technology includes new variants of CRISPR-Cas proteins from metagenomic datasets isolated from different environmental and microbiome environments that are distantly related to other CRISPR-Cas systems that utilize a guide RNA (gRNA) to perform RNA-directed cleavage of nucleic acids that can be applicable for RNA editing, diagnostics, and more. The enzyme is activated by the binding of an RNA target complementary to the spacer sequence, in order to activate cis-cleavage of the RNA target and/or unleash trans-cleavage activity against RNA substrates. This invention is especially useful for transcriptome editing as well as detecting RNA molecules, but can also detect DNA substrates upon transcription.
Suggested uses
Possible applications of this invention include:
- transcript knockdown
- transcriptome editing by fusions to ADAR proteins
- detecting RNA molecules
- detection of DNA substrates upon transcription
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Keywords
CRISPR, Cas, transciptome editing
Additional Technologies by these Inventors
- COMPOSITIONS AND METHODS FOR IDENTIFYING HOST CELL TARGET PROTEINS FOR TREATING RNA VIRUS INFECTIONS
- Genome Editing via LNP-Based Delivery of Efficient and Stable CRISPR-Cas Editors
- Type III CRISPR-Cas System for Robust RNA Knockdown and Imaging in Eukaryotes
- Cas12-mediated DNA Detection Reporter Molecules
- Highly Multiplexed Tagging Methods for RNA Imaging and Other Applications
- Improved guide RNA and Protein Design for CasX-based Gene Editing Platform
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- RNA-directed Cleavage and Modification of DNA using CasY (CRISPR-CasY)
- CasX Nickase Designs, Tans Cleavage Designs & Structure
- In Vivo Gene Editing Of Tau Locus Via Liponanoparticle Delivery
- Methods and Compositions for Modifying a single stranded Target Nucleic Acid
- A Dual-RNA Guided CasZ Gene Editing Technology
- Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF (“Cas-VariPhi”)
- A Protein Inhibitor Of Cas9
- RNA-directed Cleavage and Modification of DNA using CasX (CRISPR-CasX)
- Compositions and Methods for Genome Editing
- Split-Cas9 For Regulatable Genome Engineering
- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
- Minimal RNA Targeting CRISPR Cas Systems
- Variant Cas12a Protein Compositions and Methods of Use
- CRISPR CASY COMPOSITIONS AND METHODS OF USE
- Single Conjugative Vector for Genome Editing by RNA-guided Transposition
- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
- Engineered/Variant Hyperactive CRISPR CasPhi Enzymes And Methods Of Use Thereof
- Methods Of Use Of Cas12L/CasLambda In Plants
- Type V CRISPR/CAS Effector Proteins for Cleaving ssDNA and Detecting Target DNA
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- CRISPR-Cas Effector Polypeptides and Methods of Use Thereof
- Virus-encoded DNA-binding Proteins
- Class 2 CRISPR/Cas COMPOSITIONS AND METHODS OF USE
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Methods and Compositions for Controlling Gene Expression by RNA Processing
