Miniature Type VI CRISPR-Cas Systems and Methods of Use

Tech ID: 32413 / UC Case 2021-183-0

Patent Status

Patent Pending

Brief Description

This technology includes new variants of CRISPR-Cas proteins from metagenomic datasets isolated from different environmental and microbiome environments that are distantly related to other CRISPR-Cas systems that utilize a guide RNA (gRNA) to perform RNA-directed cleavage of nucleic acids that can be applicable for RNA editing, diagnostics, and more. The enzyme is activated by the binding of an RNA target complementary to the spacer sequence, in order to activate cis-cleavage of the RNA target and/or unleash trans-cleavage activity against RNA substrates. This invention is especially useful for transcriptome editing as well as detecting RNA molecules, but can also detect DNA substrates upon transcription.

Suggested uses

Possible applications of this invention include:

  • transcript knockdown
  • transcriptome editing by fusions to ADAR proteins
  • detecting RNA molecules
  • detection of DNA substrates upon transcription

Advantages

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

CRISPR, Cas, transciptome editing

Categorized As

Additional Technologies by these Inventors