Cas9 Variants With Altered DNA Cleaving Activity

Tech ID: 24492 / UC Case 2015-054-0

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180298360 10/18/2018 2015-054
European Patent Office Published Application WO 2016/196655 12/08/2016 2015-054
Patent Cooperation Treaty Published Application WO2016196655 12/08/2016 2015-054
 

Brief Description

RNA-programmed Cas9 has proven to be a versatile tool for genome engineering in multiple cell types and organisms.  Guided by a dual-RNA complex or a chimeric single-guide RNA, Cas9 (or variants of Cas9) can generate site-specific double-stranded DNA breaks (DSBs) or single-stranded breaks (SSBs) within target nucleic acids.  Target nucleic acids can include double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) as well as RNA.  When cleavage of a target nucleic acid occurs within a cell, the break in the target nucleic acid can be repaired by non-homologous end joining or homology directed repair.  UC Berkeley researchers have created new Cas9 protein variants, nucleic acids encoding the variant Cas9 proteins, and host cells comprising the nucleic acids.

Suggested uses

  • Genome editing
  • Gene therapy 

 

Advantages

  • Enhances CRISPR targeting specificity

 

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

CRISPR, Cas9, variants, genome, gene

Categorized As