Cost-effective Method to Quickly Produce and Purify Large Quantities of Biologically Active ncRNAs

Tech ID: 24318 / UC Case 2014-279-0


Researchers at the University of California, Davis have developed a method to develop and purify large quantities of ncRNA for basic and translational research, as well as for therapeutics delivery.

Full Description

The discovery of the role of chimeric, non-coding, RNAs (ncRNA) in the control of various cellular processes has greatly expanded researchers’ understanding of cellular genetics. Advances include insights into developing novel therapeutic strategies. As one example, tumor-suppressive ncRNAs that are depleted in carcinoma tissues may be reintroduced into cancer cells to manage tumor progression. Unfortunately, existing methods of ncRNA production - such as chemical synthesis - are very expensive and run the risk of altering the RNA’s structure and its associated biological activity. The lack of an efficient method for producing large quantities of inexpensive, naturally-occurring and biologically-functional ncRNA agents hinders basic research on ncRNA structures. It also limits potentially critical, translational research on ncRNA-based therapies.



Researchers at the University of California, Davis have developed a method to cost-effectively design, produce, and purify large amounts of chimeric, non-coding, RNA agents using recombinant RNA technology. This method involves using E.coli and tRNA scaffolding. This production approach allows for high levels of ncRNA expression and stability in a recombinant host cell. The resulting ncRNA can be purified with high homogeneity, and used to help control biological processes. The ncRNA can also become a “stealth” carrier for the delivery of therapeutic RNA agents or RNA biomarkers. 


•    Increases availability of ncRNA for basic and translational research
•    Potential carrier of biomarkers or therapeutics


•    Provides a source of inexpensive, high-purity, ncRNA
•    Increases opportunities for the delivery of therapeutic or biomarkers using stealth RNA carriers

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,619,156 04/14/2020 2014-279


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  • Chen, Qiuxia
  • Li, Meimei
  • Wang, Weipeng
  • Yu, Aiming

Other Information


Biomarkers, Chimeric RNA, E. coli, RNA delivery, RNA production, RNA purification, Therapeutics

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