New Cross-Linking Mass Spectrometry Platform: SDASO-L, SDASO-M, and SDASO-S

Brief Description

An innovative mass spectrometry platform that utilizes sulfoxide-containing MS-cleavable heterobifunctional photoactivated cross-linkers to enhance protein structural elucidation.

Applications

Structural elucidation of protein complexes

Proteomics and protein research

Development of new MS-cleavable cross-linker derivatives

Creation of a comprehensive protein-protein interaction map of cellular systems

Advantages

Enhances protein structural elucidation

Improves the detection and identification of photocross-linked peptides

Overcomes limitations of residue-specific cross-linkers

Nonspecific nature allows capturing inaccessible regions

Problems Solved:

• Difficulty in mapping interaction regions lacking targetable residues
• Complexity and low abundance of cross-linked products complicating MS analysis and database search

Full Description

This technology introduces three novel sulfoxide-containing cross-linking reagents: SDASO-L, SDASO-M, and SDASO-S. They aid in identifying protein interaction interfaces that are typically resistant to crosslinking due to a lack of targetable residues. The introduced linkers, SDASO-L (long), -M (medium), and -S (short), are created to facilitate the identification of photocross-linked peptides, thereby enabling photocrosslinking of complex samples.

Patent Status