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Development of Highly Sensitive Excitation Ratiometric Indicators of Cellular Phosphorylation

Protein phosphorylation is one of the most common forms of post-translational modification and is involved in the regulation of key signaling pathways in the cell. Dysfunctional phosphorylation plays a key role in various diseases, especially cancer and neurodegenerative disorders. Protein kinases have been the focus of intense recent interest by the pharmaceutical industry. Indeed, most new cancer drugs approved by the FDA in the last several years target kinases, and there are hundreds of new kinase inhibitors under development.  To this end, it is very important to have quantitative methods for measuring changes in kinase activities.  In vitro kinase activity assays take the target molecules out of cellular contexts. Fluorescent protein-based kinase biosensors have enabled the real-time monitoring of kinase activities within the native context of living cells, yet most commonly used biosensors exhibit poor sensitivity (e.g., dynamic range) for imaging physiological signaling activities in situ.

A Novel Method for the Isolation and Purification of Extracellular Vesicles (ECV) for Use as Potential Biomarkers

Extracellular vesicles (ECV) reflect the physiological or pathological condition of the cell. Therefore, they have emerged as potential biomarkers for disease. They can be obtained from a variety of body fluids, particularly urine that is an ideal source because it can be obtained in great quantities, recurrently and with minimal intervention. However, the characterization of urine ECV is challenging because the preparation is usually contaminated with soluble proteins. Therefore, approaches to obtain cleaner preparations of urine ECV are important for any proteomic analysis.

Biomarkers Based On Molecular Composition Of Cells

UCLA researchers in the Department of Materials Science and Engineering have developed a novel biomarker based on spectroscopic analysis of proteins in cell membranes.

Predictive Optimization Of Pharmeceutical Efficacy

UCLA researchers in the Department of Mechanical and Aerospace Engineering have developed a machine learning platform to virtually screen combinatorial drug therapies.

Living Bioreactor for Stoichiometric Protein Production

Living bioreactors are powerful systems for producing a variety of valuable compounds. The versatility of such bioreactors is one of the more useful aspects of the system. Large quantities of compounds or cellular components can be produced efficiently, with minimal cost. Alternately, these systems can be used to produce pathway components that are necessary in the production of secondary products. A common problem with such systems is that they are limited by non-uniform production of pathway components, or require an isolation process to ensure the components are in the appropriate quantity and sequence in the process. Inventors at Texas A&M and UC San Francisco have developed a novel technique to address these issues. The technology effectively results in a stoichiometric production of protein components that are produced in an array, ready for secondary production.

Rapid Screening and Identification of Antigenic Components in Tissues and Organs

Researchers at the University of California, Davis have developed an approach to rapidly screen and identify antigenic components in tissues and organs.

Versatile Labeling of Protein N-Termini for Site-specific Bioconjugation

Improved subtiligase variants allow broad and versatile site-specific chemical modification or conjugation of proteins on their N-termini.

Determination Of Absolute Configuration Of Secondary Alcohols Using A Competing Enantioselective Conversion Kit

The absolute configuration of an organic compound dictates its interactions with other chemicals. The Competing Enantioselective Conversion (CEC) method is an attractive method for determining the absolute configuration of secondary alcohols, but the preparation of stock reagent solutions takes longer than the analysis time itself – a mere 1-2 hours. The inventors at UCI have developed a CEC kit which contains stock solutions of the components required for CEC that remain stable and usable for several months.

Aptamer functionalized shrink-induced high surface area electrochemical sensors

A low-cost method of manufacturing a, rough high surface area electrodes with a dissolvable polymer coating to improve surface wettability and electrochemical sensing.

Assay for Inhibitors of Nonsense-Mediated RNA Decay

Prof. Sika Zheng at UCR has developed a new endogenous NMD assay that is both sensitive and quantitative. The assay can be used on its own to assess changes in cellular NMD activity with high specificity and sensitivity. It can facilitate analysis of NMD controls by cellular pathways in response to stimuli or during development and is particularly suitable for unbiased screening of NMD modulators. The assay is designed to distinguish NMD regulation from transcriptional regulation and alternative splicing control.

Process For Sorting Dispersed Colloidal Structures

Researchers from the Chemistry and Biochemistry department at UCLA have developed method of separating and/or sorting specific target structures from other non-target structures in a complex mixture using custom-made target-specific colloidal particles.

Drop-Carrier Particles For Digital Assays

UCLA researchers in the Department of Bioengineering have developed a novel drop-carrier particle for single cell or single molecule assays.

Low Cost Wireless Spirometer Using Acoustic Modulation

The present invention relates to portable Spirometry system that uses sound to transmit pulmonary airflow information to a receiver.

Microfluidic Component Package

The present invention describes a component package that enables a microfluidic device to be fixed to a Printed Circuit Board (PCB) or other substrate, and embedded within a larger microfluidic system.

Metal-free affinity media/agents for the selective capture of histidine-rich peptide sequences

The present invention utilizes metal-free synthetic polymer-based materials for the purification of peptides and proteins containing or being fused with histidine-rich sequences, which does not damage the function of the target protein and is less costly.

Improved Cell-Free Protein Synthesis For Protein Microarray

Researchers at UC Irvine have developed a cell-free (CF) protein synthesis system to solubilize and synthesize highly hydrophobic membrane proteins that would typically aggregate using current CF synthesis systems. With such high amounts of synthesized proteins, researchers intend to build protein microarrays for diagnostic purposes.

Enhanced Cell/Bead Encapsulation Via Acoustic Focusing

The invention consists of a multi-channel, droplet-generating microfluidic device with a strategically placed feature. The feature vibrates in order to counteract particle-trapping micro-vortices formed in the device. Counteracting these vortices allows for single particle encapsulation in the droplets formed by the device and makes this technology a good candidate for use in single cell diagnostics and drug delivery systems.

Microfluidic Pressure Regulator For Robust Hydrogel Loading Without Bursting

This invention is aimed at controlling the pressure in 3D cell cultures. It consists of a combination of microfluidic channels, which surround the extracellular matrix (ECM), tunable pressure-regulated valves, which activate when a threshold pressure is reached in the ECM, and a repository, to direct excess gel away from the cell culture if the threshold pressure is exceeded. It can prevent leakage of gel between adjacent cell cultures in high-throughput arrays and is compatible with various cell culture materials and injection equipment.

A Protein Domain That Protects Ubiquitinated Forms Of Proteins From Degradation In Cis And In Trans

Ubiquitylation affects proteins in many ways, such as activation or inactivation, and signaling for their degradation. It is not fully understood how ubiquitin effects all proteins or how researchers may use it to control cellular processes. This invention describes novel fusion proteins that protect ubiquitylated forms of the target proteins from degradation.

Novel method for detection of O-Sulfonation sites on post-translationally modified proteins

Sulfonation of proteins and carbohydrates plays an important role in signaling, transport, and metabolism in the body. The degree to which a molecule is modified and at what positions dictates how that structure interacts within the body. UCI researchers have developed novel methods of detecting and mapping serine and threonine sulfonation of peptides and proteins.

Functionally Selective Ligands for Study and Inhibition of Inflammation

Background: Due to the complexity of the complement system cascade, biological roles of many signaling receptors are unknown. Additionally, biased ligand binding to cell-bound receptors may lead to selective intracellular effector binding and ligand-specific pathway activation and function. Mechanistic knowledge forms the basis for assay development to explore pharmacology against complement-mediated inflammatory diseases.   Brief Description: A multidisciplinary team of researchers from UCR, Texas A&M, Sheffield, and Queensland have discovered the first functionally selective peptide ligands for a complement system receptor that is involved in inflammation. The peptides are functionally selective ligands of C5aR2 but not C5aR1 or C3aR, and they have been characterized in vitro and in vivo. These peptides are novel tools that can modulate the activity of the receptor in vitro and in vivo, and interrogate the function of the receptor and its implication in inflammatory diseases.

Pyrite Shrink-Wrap Laminate As A Hydroxyl Radical Generator

The invention is a diagnostic technology, as well as a research and development tool. It is a simple, easy to operate, and effective platform for the analysis of pharmaceuticals and biological species. Specifically, this platform generates hydroxyl radicals for oxidative footprinting – a technique commonly employed in protein mapping and analysis. The platform itself is inexpenisve to fabricate, scalable, and requires nothing more than an ordinary pipet to use. In addition, it is highly amenable to scale-up, multiplexing, and automation, and so it holds promise as a high-throughput method for mapping protein structure in support of product development, validation, and regulatory approval in the protein-based therapeutics industry.

Monoclonal Antibody Against Cer164 (Clone 11)

Mouse monoclonal antibody against the human centrosomal protein 164kDa (Cep164). This antibody binds to the phosphorylation site of Cep164 and has been tested for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.

Monoclonal Antibody Against ATR-IP (Clone 5)

Mouse monoclonal antibody against the human ATR-interacting protein (ATR-IP). This antibody has been tested for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.

Monoclonal Antibody Against Cer164 (Clone 26)

Mouse monoclonal antibody against the human centrosomal protein 164kDa (Cep164). This antibody binds to the phosphorylation site of Cep164 and has been tested for use in immunocytochemistry/immunofluorescence, immunoprecipitation, and western blot.

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