New Sulfoxide-Containing MS-Cleavable Cross-Linker for Proteomics

Brief Description

An innovative sulfoxide-containing MS-cleavable cross-linker, DBrASO, specifically designed for cysteine residues and aimed at enhancing protein-protein interactions studies and protein complexes architecture analysis.

Applications

Studies of protein-protein interactions

Protein complex architecture elucidation

Complementing existing lysine-reactive cross-linkers in proteomics studies

Potential utility in quantitative XL-MS studies

Advantages

Specific target on cysteine residues

Improved specificity at physiological pH

Non-hydrolyzable bromoacetamide groups

Production of homogeneous cross-linked products

Effective for global XL-MS analysis

Problems Solved:

• Fills the existing gap in XL-MS analysis
• Enables unambiguous identification of cross-linking by carrying the same MS-cleavable
• characteristics as other sulfoxide-containing MS-cleavable cross-linkers
• Overcomes the issue of hydrolyzability associated with other cross-linkers

Description

This technology involves the development of DBrASO, a new sulfoxide-containing, MS-cleavable, cysteinereactive cross-linker. It features bromoacetamide groups providing improved specificity, non-hydrolyzability and homogeneous cross-linked products. The product is designed for unambiguous cross-link identification and has been seen to effectively work on simple and complex samples. DBrASO represents a breakthrough product in proteomics studies, offering a comprehensive solution for protein interaction mapping on a systems-level basis.

Patent Status