BMSO: A Novel Sulfoxide-Containing Cleavable Cysteine Crosslinker

Brief Description

BMSO represents a groundbreaking advancement in crosslinking mass spectrometry (XL-MS), enabling comprehensive mapping of protein-protein interactions.

Full Description

BMSO, or bis-maleimide sulfoxide, is a cutting-edge cysteine-reactive crosslinker designed to enhance the identification of cysteine crosslinked peptides in protein-protein interaction (PPI) studies. By incorporating a sulfoxide-containing spacer arm that is mass spectrometry (MS)-cleavable, BMSO enables straightforward and unambiguous identification of crosslinked peptides, significantly advancing the capabilities of crosslinking mass spectrometry (XL-MS) for studying complex protein architectures and interactions.

Suggested uses

• Proteomics research for detailed protein interaction mapping. 
• Structural biology for elucidating protein complex architectures. 
• Drug discovery and development for identifying potential therapeutic targets. 
• Biomedical research for understanding disease mechanisms at the molecular level.

Advantages

• First-generation cysteine-reactive crosslinker with mass spectrometry (MS) cleavability. 
• Facilitates unambiguous identification of cysteine cross-linked peptides. 
• Enables comprehensive mapping of protein-protein interactions (PPIs). 
• Complements existing lysine- and acidic residue-targeting crosslinkers. 
• Performs well in complex biological samples due to MS-cleavable design.

Patent Status

Related Materials

• Gutierrez, C. B., et al. Rychnovsky, S. D., Huang, L. (2018). Development of a Novel Sulfoxide-Containing MS-Cleavable Homobifunctional Cysteine-Reactive Cross-Linker for Studying Protein–Protein Interactions. Anal. Chem. 90 (12).