Cell expansion for cartilage tissue production usually leads to loss of the potential to produce cartilage, which impedes uses for cartilage repair. This invention features methods and systems for producing highly expanded primary cells to construct functional neocartilage and other neotissue. According to a 2015 global market report, tissue engineering technologies are expected to reach over 94B USD by 2022.
UCI scientists have discovered methods and compositions for producing highly expanded cells with an ability to form neocartilage. The method of cell expansion entails culturing the cells from cadaveric tissue in a cocktail of growth factors and differentiating agents, and culturing in 3-D the expanded cells for redifferentiation in a redifferentiation cocktail. The resulting engineered cells have functional properties comparable to native cartilage and the potential to produce neocartilage. Notably, the invention enables a large quantity of engineered cartilage implants to be produced from a few cells.
In vitro evaluation of native and engineered tissues using cadaveric cartilage Assessed biomechanical and biochemical properties
· Neotissue production (e.g., neocartilage)
· Tissue-engineered cartilage-like tissue (TCL) treatment
· Tissue engineering applications