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Intracellular-Ligand-Responsive Cytotoxic Molecules For Selective T-Cell Mediated Cell Killing

UCLA researchers in the Department of Chemical and Biomolecular Engineering have developed a novel immunotherapeutic strategy that uses a selectively-activated cytotoxic molecule to enable tumor-specific T cell-mediated killing.

Inhibition Of Protein Tyrosine Phosphatase - Sigma For Hematopoietic Regeneration

UCLA Researchers have identified a novel pharmacological target for hematopoietic stem cell regeneration. They have developed small molecule inhibitors against the target and shown that the inhibitors cause rapid stem cell regeneration.

Xenobiotic-Free Culture System To Expand Human Limbal Stem Cells

UCLA researchers in the Departments of Opthalmology have developed a xenobiotic-free manufacturing process to produce transplantable human limbal stem cells for use in treating limbal stem cell deficiency.

Microchambers With Solid-State Phosphorescent Sensor For Measuring Single Mitochondrial Respiration

The invention is a miniaturized device that assays the respiration of a single mitochondrion. Through a novel approach for measuring oxygen consumption rate, the device provides information on cell and tissue mitochondrial functional. This data is relevant for understanding human conditions associated with mitochondrial dysfunction, such as Alzheimer’s Disease and cancer.

A Method for Induction of Corneal Endothelial Cells from Human Pluripotent Stem Cells (PSCs) via Ocular Lineage Specification

One of the most common causes of loss of vision is by corneal endothelial dystrophy (CED). Moreover, Fuchs CED is the leading cause of age-related blindness in individuals over the age of 40 in the United States affecting ~ 4% of the population. The current standard of care is to perform restorative corneal transplantation, but due to a shortage of healthy human donors, this is a challenge confronting the medical community. One solution would be to develop alternative sources of transplantation material. Human corneal endothelial cells (CESs) are not proliferative and do not regenerate in vivo. Therefore, there is a major interest in development of in vitro expandable cell sources for engineering corneal endothelium.

Culturing More Mature iPSC-derived Cardiac Myocytes

Researchers at the University of California, Davis have developed a non-genetic, non-pharmacological method for culturing more mature induced pluripotent stem cell-derived cardiac myocytes.

Small Molecule Generation of Multinucleated and Striated Myofibers from Human Pluripotent Stem Cells Equivalent to Adult Skeletal Muscle

Researchers in the UCLA Department of Microbiology, Immunology and Molecular Genetics have developed a novel means of generating adult skeletal muscle-equivalent myofibers from human pluripotent stem cells.

CRISPR/Cas9 Mediated Genome Editing For Duchenne Muscular Dystrophy

UCLA researchers in the Department of Microbiology, Immunology & Molecular Genetics have developed a method to permanently correct the out-of-frame dystrophin gene in patient cell models of Duchenne Muscular Dystrophy (DMD)

Induced Pluripotent Stem Cell-Derived Glial Enriched Progenitor Cells For The Treatment Of White Matter Stroke

UCLA researchers in the Department of Neurology and the Department of Molecular, Cell & Developmental Biology have developed novel therapies for cerebral ischemic injuries, including white matter stroke, using glial-enriched progenitor cells.

Identification Of A Factor That Promotes Human Hematopoietic Stem Cell Self-Renewal

The Mikkola group at UCLA has discovered a novel regulator of hematopoietic stem cell self-renewal. The overexpression of this regulator increases the yield of ex vivo stem cell expansion and could thereby improve the efficiency of stem cell therapies. 

CARDIAC TISSUE MODELS AND METHODS OF USE THEREOF

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Tissue engineering approaches have the potential to increase the physiologic relevance of human iPS-derived cells, such as cardiomyocytes (iPS-CM). However, forming Engineered Heart Muscle (EHM) typically requires >1 million cells per tissue. Existing miniaturization strategies involve complex approaches not amenable to mass production, limiting the ability to use EHM for iPS-based disease modeling and drug screening. Micro-scale cardiospheres are easily produced, but do not facilitate assembly of elongated muscle or direct force measurements.   UC Berkeley researchers have developed a 3D filamentous fiber matrix that combines features of EHM and cardiospheres: Micro-Heart Muscle (μHM) arrays, in which elongated muscle fibers are formed in an easily fabricated template, with as few as 2,000 iPS-CM per individual tissue. Within μHM, iPS-CM exhibit uniaxial contractility and alignment, robust sarcomere assembly, and reduced variability and hypersensitivity in drug responsiveness, compared to monolayers with the same cellular composition. μHM mounted onto standard force measurement apparatus exhibited a robust Frank-Starling response to external stretch, and a dose-dependent inotropic response to the β-adrenergic agonist isoproterenol.  

Mesenchymal Stem Cell Derived Exosomes for Treating Peripheral Artery Disease

Researchers at the University of California, Davis have developed a method to isolate exosomes from mesenchymal stem cells that contain signaling molecules that induce angiogenesis. The isolated exosomes can be used for treating peripheral arterial disease.

Microfluidic Pressure Regulator For Robust Hydrogel Loading Without Bursting

This invention is aimed at controlling the pressure in 3D cell cultures. It consists of a combination of microfluidic channels, which surround the extracellular matrix (ECM), tunable pressure-regulated valves, which activate when a threshold pressure is reached in the ECM, and a repository, to direct excess gel away from the cell culture if the threshold pressure is exceeded. It can prevent leakage of gel between adjacent cell cultures in high-throughput arrays and is compatible with various cell culture materials and injection equipment.

Discriminating Naive Human Pluripotency

UCLA Researchers in the Department of Molecular, Cell, and Developmental Biology have developed a simple molecular approach to non-invasively distinguish and isolate human pluripotent stem cells that have reverted from the primed pluripotent state to the native state.

Compositions and Methods for Inhibiting Stem Cell Aging

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} By 2050 the number of people in the world that will be aged 65 or older is expected to nearly triple to about 1.5 billion, representing 16% of the world’s population. One aspect of aging involves a diminished capacity to repair tissues after injury. This diminished capacity is evident in certain conditions that occur with aging, such as anemia, sarcopenia (loss of muscle mass), and osteoporosis. Deterioration of adult stem cells accounts for much of aging-associated compromised tissue maintenance. Adult stem cells mostly reside in a metabolically inactive quiescent state to preserve their integrity, but convert to a metabolically active proliferative state to replenish the tissue. The signals that trigger stem cells to exit the cell cycle and re-enter quiescence, and the signal transduction leading to the transition remain elusive however.   UC Berkeley researchers have developed methods of reducing or inhibiting or reversing stem cell aging or preventing and/or reversing tissue degeneration or injury by increasing the activity and/or the level of SIRT2 in an adult stem cell or reducing the level of a nucleotide-binding domain and leucine-rich repeat- containing-3 (NLRP3) polypeptide in an adult stem cell.  

Novel method for detection of O-Sulfonation sites on post-translationally modified proteins

Sulfonation of proteins and carbohydrates plays an important role in signaling, transport, and metabolism in the body. The degree to which a molecule is modified and at what positions dictates how that structure interacts within the body. UCI researchers have developed novel methods of detecting and mapping serine and threonine sulfonation of peptides and proteins.

A non-destructive method of quantifying mRNA in a single living cell

The detection of levels of messenger RNA (mRNA), the molecule used by DNA to convey information about protein production, is a very important method in molecular biology. Current detection strategies, such as Northern Blotting and RT-PCR, require destruction of the cell to extract such information. Researchers at the University of California, Irvine have developed a method to non-destructively assess mRNA levels in a single living cell.

CHEMICAL INDUCTION OF A PAUSED PLURIPOTENT STATE

This invention identifies a mechanism for pausing development of pre-implantation embryos while retaining viability.

Methods For Promoting Oligodendrocyte Regeneration And Remyelination

Researchers at the University of California Davis have demonstrated that immature astrocytes generated from human pluripotent stems cells, promote oligodendrocyte lineage progression via TIMP-1 secretion.

New Tools To Detect, Track And Target Cancer Cells In Vivo

The researcher has developed a novel fluorescent reporter mouse in which fluorescent signals reflected endogenous Msi expression (Msi1eYFP, Msi2eGFP, Msi1 reporter mice (Reporter for Musashi1, or REM1) showed expression in the stem cell enriched adult subventricular zone, and Msi1+ cells were Nestin+ and CD133+  consistent with Msi1 marking neural stem/progenitor cells. Msi2 reporters (REM2) reflected endogenous expression of Msi2, being  highest in hematopoietic stem cells and declining with maturation.The Msi reporters described here represent exciting new tools that could be broadly useful for studying cancer. Because Msi reporter activity can be visualized through live imaging these reporter mice can be uniquely used to image and track cancer stem cells in vivo, and can provide a dynamic view of endogenous cancer growth, tumor dissemination and metastasis in its native microenvironment.  The fact that reporter positive cells are preferentially gemcitabine resistant, raises the exciting possibility that this could serve as a new platform to identify therapy resistance in vivo. The integration of such reporters in drug development may provide a powerful and sophisticated complement to traditional screens, by allowing the identification of therapies that are better able to target tumor propagating cells, and drug resistant residual disease. In addition, the spatially restricted distribution of Msi+ cells could have important implications for loco regional, aggressive targeting of driver cells that mediate resistance and disease relapse.

Deriving Human Naïve Pluripotent Stem Cells by Modifying the Hippo Pathway Using Genetic or Chemical Approaches

This invention identifies a method of generating naïve pluripotent stem cells for subsequent use in research or for regenerative medicine.

Stem Cell Treatment for Oral Inflammatory Disease and Biomarker to Predict Response

Researchers at the University of California, Davis have developed a stem cell therapy to treat chronic, oral inflammatory disease and a biomarker for predicting whether there will be a response to therapy.

Use of Embryonic Stem Cell-Specific microRNAs to Safely Promote Induced Pluripotency

Novel use of a family of microRNAs to promote the de-differentiation of somatic cells to induce pluripotent stem cells (iPS cells) for use as therapeutic agents or research tools.

Generation Of Human Beta Cell Equivalents From Pluripotent Stem Cells In Vitro

This invention describes a robust method to generate functional human beta cell equivalents from pluripotent stem cells in vitro for wide applications in basic research, drug and toxicology screens and as a diabetes cell therapy.

Novel Hydrogel for Optimized Cell Delivery, Culture and Inflammation Prevention from De-cellularized Human Amniotic Membrane

A novel, human amnion derived hydrogel has been shown to considerably optimize cell delivery and scaffolding by increasing cellular survival, proliferation, and integration, as well as significantly decreasing host rejection and morbidity.

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