Current methods for cell encapsulation into droplets are inefficient and result in empty and unusable droplets. Researchers at UC Irvine have invented a novel microfluidic device capable of encapsulating cells at a very high efficiency. This tool can be used as a research tool for sorting and classifying cells for further downstream cell assays.
Cell encapsulation into droplets is an integral part of droplet microfluidics. Improved cell encapsulation into the droplets is necessary as current methods for cell loading into droplets are inefficient, resulting in a majority of empty and unusable drops. Researchers at UC Irvine have invented a novel microfluidic system for generating droplets with highly efficient cell encapsulation.
The presence of objects such as cells in a fluid changes the electrical properties of the fluid. The invented system integrates impedance measurements with on-demand droplet generation process to enable selective generation of droplets when the presence of an object such as a cell is detected. This ensures that a high percentage of droplets that are generated actually contain the object and addresses the persistent problem of empty droplets. The impedance sensor not only determines object presence, but also can determine the size, shape of the object, and speed at which the object is moving.
Figure 18 shows a graph of cell impedance data that is used for impedance signal processing. The present invention is able to distinguish object size and speed and use that information to generate the appropriate sized droplet.
Figure 20 illustrates the encapsulation control scheme of the invention. Following impedance, or detection of cell presence, the chamber is activated for cell encapsulation.
The present invention has been shown to be able to accommodate densities of 1x106 cells/mL.
This microfluidic system can be used as a droplet encapsulation device. It can also be used for selective extraction of precise, digitized volumes out of a larger, continuous volume of fluid. Since the invention utilizes an impedance-based detector, this invention can also be used to select for cells with unique impedance signatures, for example, encapsulating white blood cells from whole blood.
The system senses changes in electrical properties of the fluid when objects such as cells are present, and generates droplets when cell presence is confirmed. This ensures a highly efficient process that can withstand a high density of cells. This process can distinguish between sizes and speed of object type and account for differences by changing the size of the produced droplet.
|United States Of America||Issued Patent||9,999,886||06/19/2018||2011-162|
microfluidics, cell encapsulation, cell sorting, droplets