Researchers at the University of California, Davis (UC Davis) have developed a simplified procedure to synthesize complex glycosphingosine compounds for the chemical preparation of glycosphingolipids.
Glycosphingolipids, a sugar-conjugated lipid, are found in the cell membranes of organisms from bacteria to humans and are used in a range of biological processes such as proteinsorting,signaltransduction,membranetrafficking,viral/bacterialinfection, and cell-to-cell communications.Obtaining pure glycosphingolipid products to evaluate their biological capabilities/function has proved challenging. Techniques to synthesize synthetic glycosphingolipids have been developed using a variety of chemical procedures. However, they have proven to be complicated to carry out and often result in low product yields due to their complexity.
Researchers at UC Davis have developed an improved process to prepare glycosphingolipids using a simplified strategy whereby glycosphingosines are produced/used as an intermediary for creating glycosphingolipids.The technique allows for the efficient synthesis of glycosphingosines and glycosphingolipids, which can be easily cleansed by adding a commercially available detergent, thereby increasing reaction efficiency, and purified/filtered using commercially available solid extraction cartridges. The developed synthesis technique increases reaction dynamics and allows modifying the created glycosphingosines and glycosphingolipids by adding sugars and fats to evaluate the product's potential for new medical applications.