Substrate And Process Engineering For Biocatalytic Synthesis And Facile Purification Of Human Milk Oligosaccharides (HMOs)

Tech ID: 33436 / UC Case 2022-559-0

Abstract

Researchers at the University of California, Davis have developed an innovative method for efficient, high-yield production and easy purification of Human Milk Oligosaccharides (HMOs) using a Multistep One-Pot Multienzyme (MSOPME) process.

Full Description

Researchers at the University of California Davis have developed a process that couples substrate and process engineering for the synthesis and purification of structurally defined HMOs. It employs a glycosyltransferase acceptor substrate-tagging strategy, where the carboxybenzyl (CBz) tag simplifies the purification process via the C-18 column and can be easily removed post-purification. This MSOPME process enables production of complex targets without intermediate oligosaccharides' isolation, with a single C18-cartridge purification process of the final product.

Applications

  • Production of key ingredients in products aimed at improving human health. 
  • Creation of prebiotics and bacteriostatic agents. 
  • Development of nutrients for brain development. 
  • Production of potential nutraceuticals and therapeutics. 
  • Supplement for infant formulas.

Features/Benefits

  • Efficient synthesis of complex HMOs without the need for intermediate oligosaccharides purification. 
  • Facilitated HMO product purification using a single C18 cartridge/column through the use of a CBz tag. 
  • Easy removal of CBz tag to form desired HMOs with a free reducing end. 
  • Ability to synthesize structurally defined HMOs in preparative and gram scales. 
  • Environmentally friendly enzymatic production method. 
  • Potential to be readily adapted for automation. 
  • Solves limited access to structurally defined HMOs in sufficient quantities. 
  • Addresses need for intermediate steps in the synthesis and purification of HMOs. 
  • Overcomes challenges associated with large-scale production of HMOs.

Patent Status

Country Type Number Dated Case
Patent Cooperation Treaty Reference for National Filings WO 2023/141513 07/27/2023 2022-559
 

Patent Pending

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Inventors

  • Bai, Yuanyuan
  • Chen, Xi
  • Yu, Hai

Other Information

Keywords

biocatalysis, carbohydrate, chemoenzymatic synthesis, glycosylation, human milk oligosaccharides (HMOs)

Categorized As