Substrate And Process Engineering For Biocatalytic Synthesis And Facile Purification Of Human Milk Oligosaccharides (HMOs)

Abstract

Researchers at the University of California, Davis have developed an innovative method for efficient, high-yield production and easy purification of Human Milk Oligosaccharides (HMOs) using a Multistep One-Pot Multienzyme (MSOPME) process.

Full Description

Researchers at the University of California Davis have developed a process that couples substrate and process engineering for the synthesis and purification of structurally defined HMOs. It employs a glycosyltransferase acceptor substrate-tagging strategy, where the carboxybenzyl (CBz) tag simplifies the purification process via the C-18 column and can be easily removed post-purification. This MSOPME process enables production of complex targets without intermediate oligosaccharides' isolation, with a single C18-cartridge purification process of the final product.

Applications

• Production of key ingredients in products aimed at improving human health. 
• Creation of prebiotics and bacteriostatic agents. 
• Development of nutrients for brain development. 
• Production of potential nutraceuticals and therapeutics. 
• Supplement for infant formulas.

Features/Benefits

• Efficient synthesis of complex HMOs without the need for intermediate oligosaccharides purification. 
• Facilitated HMO product purification using a single C18 cartridge/column through the use of a CBz tag. 
• Easy removal of CBz tag to form desired HMOs with a free reducing end. 
• Ability to synthesize structurally defined HMOs in preparative and gram scales. 
• Environmentally friendly enzymatic production method. 
• Potential to be readily adapted for automation. 
• Solves limited access to structurally defined HMOs in sufficient quantities. 
• Addresses need for intermediate steps in the synthesis and purification of HMOs. 
• Overcomes challenges associated with large-scale production of HMOs.

Patent Status

Additional Patent Pending