Researchers at the University of California, Davis have developed an approach to rapidly screen and identify antigenic components in tissues and organs.
Identification of antigenic components of allograft and xenograft tissues is important in identifying proteins that potentially play a role in host rejection. Until now, researchers have relied exclusively on two-dimensional gel electrophoreses methods. Although effective, these methods have significant limitations due to the time required to run 2D gels and the subsequent need to extract and separate the proteins from duplicate gels prior to mass spectrometry analysis. These methods take at least 3 days time, are difficult to reproduce, require extensive optimization for each tissue, are inapplicable to certain protein classes, and are at best semi-quantitative.
Researchers at the University of California, Davis have developed a unique approach to rapidly screen and identify large numbers of antigenic components in tissues and organs. This method is capable of simultaneously identifying a multitude of antigenic components in allografts or xenografts and can be completed within 8 hours. It is easily reproducible, applicable to a wide range of antigens (including integral membrane proteins), provides quantitative results, and requires minimal optimization between tissue types. This method has already been tested and successfully able to identify antigenic components from native bovine pericardium xenografts.
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