A microfluidic device that measures mitochondrial membrane potential that may be used as a clinical diagnostic or a research tool.
The mitochondrial membrane potential is used to generate and regulate energy in living systems, driving the conversion of ADP to ATP, regulating ion homeostasis and controlling apoptosis, all central to human health and disease. Therefore, there is a need for tools to study its regulation in a controlled environment for potential clinical and scientific research applications.
To date, to measure mitochondrial membrane potential, researchers and technicians must use fluorescent probes or electrochemical methods such as patch clamping which has proven to be challenging. Researchers at the University of California, Irvine have developed a tetraphenylphosphonium (TPP+) selective electrode that measures the membrane potential of mitochondria and this TPP+ selective electrode may be integrated onto a microfluidic device.
This microfluidic device may be used to analyze mitochondrial bioenergetics in sample concentrations as low as 0.3 ng/μL which is four orders of magnitude smaller than the concentration used in conventional assays which use 3 μg/μL. In addition, the volume of the chamber (which is at 85 μl) is 2 orders of magnitude smaller than the volume used currently in conventional assays.
A number of prototype microfluidic devices with the unique TPP+ selective electrode have been fabricated. Mitochondrial membranes and different chemical compounds to modulate the mitochodrial membrane potential have been tested in these microfluidic devices and these microfluidic devices have shown to be sensitive and accurate in detecting the change in the mitochondrial membrane potential.
|United States Of America||Issued Patent||8,961,759||02/24/2015||2010-851|