Self-Selecting Systems For Microbiome Editing

Patent Status

Patent Pending

Brief Description

The invention is a self-selection DNA editing system for modifying microbial communities. It consists of a gene editing tool and a donor DNA with a bacteriocin unit. This unit is integrated into the target cell's genome, providing a survival advantage and ensuring that only the successfully modified cells proliferate. This allows for precise, targeted editing of microbial populations in various settings, including in vitro and in vivo environments.

Suggested uses

● Modifying a microbial community for therapeutic purposes, such as altering the human gut microbiome to treat disease.

● Editing prokaryotic cells in an industrial setting to enhance fermentation or other biological processes.

● Developing research tools for the study of microbial genetics and community dynamics.

● Creating self-selecting genetic circuits for environmental remediation, such as engineering bacteria to degrade pollutants.

Advantages

● Precision: The use of bacteriocin-based selection ensures that only the successfully modified cells survive and proliferate, making the process highly specific and efficient.

● Targeted Editing: The system allows for the precise modification of specific microbial species within a complex, mixed community.

● Enhanced Viability: The self-selection mechanism provides a significant survival advantage, leading to higher yields of the desired edited cells.

● Versatility: The system is adaptable for use in a variety of settings, including both in vitro (culture) and in vivo (natural environment) applications.

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