Puromycin Activity-Based Sensing Probes For Molecular Imaging And Histochemistry

Patent Status

Brief Description

The syntheses of the probes are highly versatile, and representative examples for detection of reactive oxygen species (ROS), reactive sulfur species (RSS), reactive carbonyl species (RCS), ROS scavengers, and redox active metal ions have been demonstrated.

One exemplary probe is Peroxymycin-1, which contains H2O2-responsive aryl boronate conjugated to puromycin through carbamate linkage. Peroxymycin-1 shows robust performance on molecular imaging of H2O2 in cell culture and histochemical analysis of H2O2 level in tissue samples harvested from small animals. It has been further employed for detection of elevated H2O2 level in liver tissues from a murine model of non-alcoholic fatty liver disease (NAFLD), suggesting its potential for studying disease pathology associated with H2O2 as well as disease diagnosis and monitoring of treatment progress.

Suggested uses

The probes are also potential tools for studying disease pathology associated with change in level of target analytes. For example, in a murine model of non-alcoholic fatty liver disease (NAFLD), one of the representative probes, Peroxymycin-1, successfully revealed an increase in H2O2 level in liver tissues.

It is anticipated that the probes can be further exploited in disease diagnosis and monitoring therapy progress by detecting target analyte level. 

Advantages

Selective and sensitive imaging of target analytes in a variety of biological samples. 

Unlike other fluorescent probes, this novel class of probes does not have intracellular leakage problems.

The probes deliver high signal-to-noise level from immunostaining.

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