The inventors at UCI have developed a method and system to make enhanced electrical impedance spectroscopy measurements in a continuously flowing train of microfluidic droplets. The technique increases the sensitivity of the electrical impedance spectroscopy measurements, lowering detection limits and increasing the frequency of continuous measurements.
Amplified detection of DNA in droplets has been demonstrated using a fluorescent probe. The use of fluorescent markers is expensive and requires extra processing steps. In addition, fluorescence intensity results must be compared to a calibration curve. Electrical impedance spectroscopy (EIS) has been used for the detection of flowing whole cells or static subcellular components.
The inventors at UCI have developed a method and system that allows the detection of a gene in genomic DNA and provides a platform that can detect DNA in flowing droplets. The technique incorporates molecular affinity forces to align molecules within droplets at the interface, resulting in increased EIS sensitivity. The method also allows for continuous measurements in traveling microfluidic droplets. The process is ideal for entities using polymerase chain reaction (PCR), as the technique is label-free and provides the capability to detect PCR amplification in many samples in real time.
Label-free detection of DNA
Nanoparticle-enhanced detection of droplet contents
Continuous amplified detection in separate droplets
Does not require fluorescent dye
|United States Of America||Issued Patent||10,203,294||02/12/2019||2014-743|
The system for both continuous microfluidic droplet generation and electrical impedance spectroscopy measurements has been developed and tested.