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Next-generation broad-spectrum anti-cancer Rad51 inhibitors

This invention describes the design, synthesis and successful evaluation of a panel of novel Rad51 inhibitors to treat a broad spectrum of cancer types.

Small Molecule Generation of Multinucleated and Striated Myofibers from Human Pluripotent Stem Cells Equivalent to Adult Skeletal Muscle

Researchers in the UCLA Department of Microbiology, Immunology and Molecular Genetics have developed a novel means of generating adult skeletal muscle-equivalent myofibers from human pluripotent stem cells.

Siderophore-Based Immunization Against Gram-Negative Bacteria

Bacterial pathogens such as E. coli and Salmonella hijack the host’s iron to cause infection. This invention describes an immunization strategy for triggering an immune response against the iron-sequestering agent secreted by the pathogen, thus turning the bacterial virulence mechanism against itself, and thereby resulting in host immunity.

Improved Cell-Free Protein Synthesis For Protein Microarray

Researchers at UC Irvine have developed a cell-free (CF) protein synthesis system to solubilize and synthesize highly hydrophobic membrane proteins that would typically aggregate using current CF synthesis systems. With such high amounts of synthesized proteins, researchers intend to build protein microarrays for diagnostic purposes.

Antibodies targeting mammalian Sterol Regulatory Element Binding Proteins (SREBP) 1 and 2

Sterol Regulatory Element Binding Proteins (SREBP) are important factors that control lipid homeostasis in mammals. Researchers at UCI have prepared antibodies that have good affinity and specificity for human SREBP1/2 for use as research tools. These antibodies have application in genetic and immunotherapeutic research areas.

Rapid And Selective Cycloaddition Reaction For Applications In Molecular Imaging

UCLA researchers in the Department of Molecular and Medical Pharmacology, and Department of Chemistry and Biochemistry have designed a new reaction with 18F-chemistry platform, allowing a highly selective, efficient and rapid approach to label biomolecules with a chemical reporter (i.e. radionuclide, fluorescent dye) for molecular imaging.

Chiral Polymers Of Intrinsic Microporosity For Membrane Separation Of Enantiomers

Many pharmaceutical drugs exist as enantiomeric pairs, chemically-distinct mirror image of one another that often exhibit marked differences in biological activity. Current methods for separating enantiomeric mixtures to generate pure form of an effective drug involve multiple time-consuming and expensive steps. The invention herein describes a polymer that can selectively separate enantiomers in a simple, continuous process.

New label-free method for direct RNase activity detection in biological samples

Researchers at the University of California, Davis have developed a new and simple, label-free method to detect milligram levels of RNase activity in undiluted biological samples that is selective, accurate and scalable

Voltage-Sensitive Dyes In Living Cells

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Comprehensively mapping and recording the electrical inputs and outputs of multiple neurons simultaneously with cellular spatial resolution and millisecond time resolution remains an outstanding challenge in the field of neurobiology. Traditionally, electrophysiology is used to directly measure membrane potential changes. While this technique yields sensitive results, it is invasive and only permits single-cell recording.  VoltageFluor dyes rely on photoinduced electron transfer to effectively report membrane potential changes in cells. This approach allows for fast, sensitive and non-invasive recording of neuronal activity in cultured mammalian neurons and in ex-vivo tissue slices. However, one major limitation of small-molecule dye imaging is the inability to target the dye to specific cells of interest.   UC Berkeley researchers have developed latent voltage sensitive dyes that require a fluorogenic activation step. This new class of VoltageFluor dyes are only weakly fluorescent until being activated in defined cell types via biological processes. In particular, the VoltageFluor dyes described herein comprise a bioreversible group that quenches the fluorescence of the VoltageFluor dye, that upon selective removal by the action of biological processes (e.g., enzymes) thereby activates the fluorescence of the VoltageFluor dye. The researchers found that the new dye facilitated the observation of spontaneous activity in rat hippocampal neurons.  

Preventing Protein Aggregation using Thermal Protectants

Protein aggregation in the brain are the causes of the neurodegenerative diseases Alzheimer’s and Parkinson’s. To study diseases and cellular mechanisms, biologists need to be able to efficiently synthesize, isolate and purify proteins. The invention herein is a synthetic nanoparticle (NP) that protects proteins from aggregation at temperatures, which normally cause aggregation. Furthermore, multiple stimuli can release the protein in high yield from the NPs.

Novel method for detection of O-Sulfonation sites on post-translationally modified proteins

Sulfonation of proteins and carbohydrates plays an important role in signaling, transport, and metabolism in the body. The degree to which a molecule is modified and at what positions dictates how that structure interacts within the body. UCI researchers have developed novel methods of detecting and mapping serine and threonine sulfonation of peptides and proteins.

Sensitive Probe for In Vitro Detection of A-beta Aggregates

The extreme sensitivity of Probe‐Enabled Fluorescence Correlation Spectroscopy (PE‐FCS) can enable significantly improved sensitivity for monitoring the aggregation of amyloidogenic proteins vs. current methods which measure the fluorescence of the amyloid‐binding dye Thioflavin T (ThT) or Thioflavin S (ThS) using bulk fluorescence measurements. A significant limitation of the conventional method is that neither ThT nor ThS fluoresce appreciably over background fluorescence until the size and number of amyloid or amyloid‐like species is quite large. Hence, one cannot detect early formed aggregate intermediates that are implicated as the most active species involved in the pathology of amyloid‐associated diseases, such as Alzheimer’s Disease.

Novel cyanobacteriochromes responsive to light in the far-red to near-infrared region

Researchers at the University of California, Davis have identified new cyanobacteriochromes (CBCRs) that detect and fluoresce in the far-red and near-infrared region of the electromagnetic spectrum.

PHOTO-INDUCED ELECTRON TRANSFER VOLTAGE SENSITIVE DYES

The development of fluorescent indicators for sensing membrane potential can be a challenge.  Traditional methods to measure membrane potential rely on invasive electrodes, however, voltage imaging with fluorescent probes (VF) is an attractive solution because voltage imaging circumvents problems of low- throughput, low spatial resolution, and high invasiveness. Previously reported VF probes/dyes have proven useful in a number of imaging contexts. However, the design scheme for VF dyes remains elusive, due in part to our incomplete understanding of the biophysical properties influencing voltage sensitivity in our VoltageFluor scaffolds.   UC Berkeley researchers have discovered new VF dyes, which are a small molecule platform for voltage imaging that operates via a photoinduced electron transfer (PeT) quenching mechanism to directly image transmembrane voltage changes.   The dyes further our understanding of the roles that membrane voltage plays, not only in excitable cells, such as neurons and cardiomyocytes, but also in non-excitable cells in the rest of the body.

Stimulus-responsive Polymers

Synthetic polymer constructs are an important tool in modern medical practice, but the lack of control over their activity limits their utility. The ability to combine structural function with localized interaction has proven extremely successful in stents, but polymer technology has not advanced sufficiently to serve a wider range of needs. PLGA polyesters can be degraded by hydrolysis facilitating their widespread use in medicine and biomedical research. Their dependence on slow hydrolysis makes for long degradation times (half-life of one year in vivo) limiting their applicability. While degradation can be sped up by copolymerization with more hydrophilic monomers; degradation is still too slow for triggered release or degradation.

Pyrite Shrink-Wrap Laminate As A Hydroxyl Radical Generator

The invention is a diagnostic technology, as well as a research and development tool. It is a simple, easy to operate, and effective platform for the analysis of pharmaceuticals and biological species. Specifically, this platform generates hydroxyl radicals for oxidative footprinting – a technique commonly employed in protein mapping and analysis. The platform itself is inexpenisve to fabricate, scalable, and requires nothing more than an ordinary pipet to use. In addition, it is highly amenable to scale-up, multiplexing, and automation, and so it holds promise as a high-throughput method for mapping protein structure in support of product development, validation, and regulatory approval in the protein-based therapeutics industry.

New Borylated Heterocycles: Indoles, Isoxazoles, Lactones, and Benzofurans, and the Methods to Make Them (related to UC Case 2013-921)

Boron building blocks play a key role in modern organic chemistry, especially in drug design and materials synthesis. Methods to generate heterocycles and borylated compounds in the same synthetic step are largely unknown; the ability to do both increases efficiency and rapidly builds molecular complexity while providing access to previously unavailable building blocks.

Method For Detecting Protein-Specific Glycosylation

O-GlcNAc modification is a common form of post-translational modification that mediates cellular activity and stem cell programming by modifying transcription factors. Multiple human diseases, including cancer and diabetes, have been linked to aberrant O-GlcNAcylation of specific proteins.Despite the importance of this modification, current methods for detection require advanced instrumentation and expertise as well as arduously enriched or purified samples. The “Glyco-seq” method developed by UC Berkeley researchers is highly sensitive, easy to use, and enables O-GlcNAc detection on proteins of interest in cell lysate. 

Novel 3D Stem Cell Culture Systems

Many disorders result in tissue degeneration, including Parkinson’s disease, heart attacks, and liver failure. One promising approach to treat these disorders is cell replacement therapy, which would implant new cells or tissues to replace those damaged by disease. Cell replacement therapy relies on stem cells, which are able to differentiate into a wide number of mature cell types. However, cell replacement therapies require large numbers of cells to clinically develop and commercialize, and the current stem cell culture methods are problematic in multiple ways, including low cell yields in 2D and poorly defined culture components. By culturing stem cells three-dimensionally, instead of two-dimensionally, far larger numbers of cells can be generated. Current three-dimensional culturing systems, however, often exert harmful shear stresses and pressures on the cells, have harsh cell recovery steps, do thus not generate large cell yields.   UC Berkeley researchers have developed new materials intended for use in fully chemically defined processes for large-scale growth and differentiation of stem cells. These materials prevent harsh cell recovery steps, and can be used in a defined, highly tunable, and three-dimensional cell culture system. 

Dielectrophoresis-Based Cell Destruction to Eliminate/Remove Unwanted Subpopulations of Cells

This invention allows for label free cell separations and cell enrichment.

Long Wavelength Voltage Sensitive Dyes

Rapid changes in the membrane potential of neurons and cardiomyocytes define the unique physiology of these cells. The classical means to monitor membrane potentials is patch clamp electrophysiology, a low-throughput and highly invasive technique. One current alternative is to use calcium imaging, as the agents are robust and sensitive, come in a variety of colors, and can be used in a wide range of biological contexts. Calcium imaging, however, allows only an imperfect approximation of membrane potential changes, and fast-spiking neuronal events are difficult to resolve. Fluorescent voltage sensors can achieve fast, sensitive, and non-disruptive direct readouts of membrane potentials. UC Berkeley researchers have designed and synthesized a new fluorophore called ‘Berkeley Red’ that can be used in the context of voltage-sensing scaffolds to generate fluorescent voltage sensors. 

Detection System for Small Molecules

There is a great demand for sensitive tests that can be used for the detection of very small analytes. Standard ELISA assays require a competitive format that loses sensitivity and produces readings inversely proportional to the analyte concentration. However, researchers at the University of California, Davis have developed an efficient and easy assay to determine the presence of very small molecule analytes such as pollutants, pesticides, drugs, toxins, and pharmaceuticals.

Low-Cost Chromatin Assembly Kit

Brief description not available

Novel Multivalent Bioassay Reagents

The guiding principle for the creation of biomolecular recognition agents has been that affinity is essential for both strength and specificity.  Monoclonal antibodies, the dominant workhorse of affinity reagents, have mono-valent affinities in the uM-nM range with apparent affinities that can be sub nM with the bi-valency intrinsic in intact immunoglobulin structure.  The avidin-biotin interaction used ubiquitously for biomolecular assembly is femto-molar and both highly specific and essentially irreversible.  High affinity has been proclaimed the essential goal for the selection of useful specific aptamers, though there has been disagreement about a tight coupling of affinity and specificity.  

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