Systems And Methods For The Preparation Of Peptide Receptive Mhc-I/Chaperone Complexes With Native Glycan Modifications

Tech ID: 33059 / UC Case 2020-251-0


Typically, peptide receptive MHC-I multimer reagents are prepared in bacterial (E. coli) culture. While this is efficient, it does not result in glycosylation of the MHC-I peptide fragments as is done in mammalian cells. As a result, if such reagents are produced in mammalian cells, proper glycosylation would result and the reagents would have a potentially more accurate representation of the natural T-cell target. 


Technology Description

This technology involves the coexpression of leucine zipper tagged single chain class I MHC molecules and the TAPBPR chaperone in mammalian cells to produce glycosylated MHC-I that are ready to accept an antigenic peptide of interest.

The expressed MHC-I can be purified from the supernatant, the leucine zippers removed through use of a specific protease (with a site engineered into the construct), multimerized, and contacted with the peptide of interest. In contrast with other technologies in this portfolio (e.g. 2018-408), no placeholder peptide is needed to create the peptide receptive MHC-I. 

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Peptide-receptive (empty) MHC-I reagents 

MHC-I Multimer reagents

T Cell receptor discovery 

T Cell epitope identification 



Glycosylated MHC-I are more realistic

Efficient production of soluble MHC-I reagents from mammalian cells

MHC-I can be purified from supernatants

No need for placeholder peptide 

Intellectual Property Information

Country Type Number Dated Case
European Patent Office Published Application 402841.2 07/20/2022 2020-251
United States Of America Published Application 20210155670 05/27/2021 2020-251
United States Of America Published Application 20210079461 03/18/2021 2018-408

Additional Patent Pending

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Other Information


MHC-I, Glycosylated MHC-I, MHC-I multimer, Empty MHC-I, Peptide receptive MHC-I, Mammalian MHC-I, MHC-I tetramer, MHC-I reagent, TAPBPR, chaperone, MHC, Major Histocompatability Complex

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