Lipoxygenases form a large family of enzymes capable of oxidizing arachidonic acid and related polyunsaturated fatty acids. One such lipoxygenase, 12/15 LOX can oxidize both the C-12 and C-15 of arachidonic acid, forming 12- or 15-hydroperosyarachidonic acid (12- or 15-HPETE). Lipoxygenases and their metabolites have been implicated in many diseases. In particular 12/15-LOX (also known as 15-LOX-1, 15-LOX, or 15-LO-1 in humans and L-12-LoX, leukocyte-type 12-LO, or L-12-LO in mice) plays a role in atherogenesis, diabetes, Alzheimer's, newborn periventricular leukomalacia, breast cancer, and stroke. Whatever the name, the protein is encoded by the gene ALOX15 in both mice and humans.
Lox inhibitors are difficult to develop due to the mouse and human homologs having different substrate and inhibitor specificities - 12/15 LOX produces predominantly 15-HETE in humans and 12-HETE in mice. So existing inhibitors are not selective for 12/15 LOX with regard to other LOX isoforms. In addition, many are strong antioxidants and therefore may result in off-target effects.
A collaboration between UC Santa Cruz, Boston Children's Hospital, and the National Institutes of Health performed a systematic chemical screen for 12-LOX inhibitors. The screen revealed a family of compounds that exhibit inhibitory effects on 12/15-lipoxygenase. Accordingly, the present invention relates to the use of these compounds for the inhibition of 12/15-lipoxygenase and for the treatment of a condition involving 12/15-lipoxygenase. Exemplary conditions include, but are not limited to, stroke, periventricular leukomalacia, cardiac arrest with resuscitation, atherosclerosis, Parkinson's disease, Alzheimer's disease, and breast cancer.
Issued patents include methods and compounds
Structure of lead compound: ML351
Potential therepeutic for:
|United States Of America||Issued Patent||10,287,279||05/14/2019||2016-385|
|European Patent Office||Published Application||3036226||06/29/2016||2016-385|
|Canada||Published Application||2015/027146 A1||02/26/2015||2016-385|
|United Kingdom||Published Application||2016-385|
12/15-LOX, 12/15-Lipoxygenase, Lipoxygenase, Arachidonic Acid, HETE, 12-HETE, 15-HETE, Diabetes, Atherogenesis, Alzheimer's, Cardiac arrest, Atherosclerosis, Parkinson's disease, Breast Cancer, Stroke, Newborn periventricular leukomalacia