High-Efficiency One-Cell-One-Bead Encapsulation In Droplets

Brief Description

A high-efficiency single-cell droplet encapsulation method to improve single cell pharmacological assay throughput.

Full Description

Background:

• Cell and bead droplet encapsulation is common technique used in heterogeneous single cell pharmacology assays probing genomics, and proteomics.
• Current solutions for one bead-one cell encapsulation are based on random encapsulation dictated by Poisson statistics.

Problem:

• Low (0.1%) efficiency of one bead-one cell droplet encapsulation limits droplet sequencing and heterogeneous assays throughput

Solution:

• Increased one bead-one cell encapsulation efficiency (> 300 times current techniques) utilizing a controllable hydrodynamic vortices microfluidic platform

Suggested uses

• Increased throughput of genome-wide expression profiles for individuals cells
• High efficiency one-bead-one cell encapsulation for droplet based heterogeneous single cell assays

Advantages

• Improved cell-bead encapsulation throughput for droplet sequencing applications of single cell genomics and proteomics
• Improving the encapsulation efficiency of droplet containing a single cell and a single bead compared to encapsulation of either component individually.

Patent Status