Nucleic Acid Tetramers For High Efficiency Multiplexed Cell Sorting
Tech ID: 27466 / UC Case 2008-685-0
UCLA researchers in the Departments of Medicine and Pharmacology have a highly specific method of sorting cells by using multiplexed tetramers with unique DNA-oligomer signatures.
The standard methods of isolating cells of interest include FACS (fluorescence-activated cell sorting) and ‘panning.’ Both methods use antibodies as a means of selecting for specific cell populations. A limitation of FACS is that the surface bound antibodies on the cell are often improperly oriented. This often leads to a decreased affinity and therefore decreased affinity for isolating cells. This makes FACS and panning unreliable techniques for isolating extremely rare cell populations.
Researchers at UCLA have developed a highly specific method of sorting cells by using multiplexed tetramers with unique DNA-oligomer signatures. This will allow users to very specifically isolate rare cells within the sample. This technique is currently being applied to isolate T cells from cancer patients for use in personalized immunotherapy.
- Cell Sorting
- In research settings
- Detection of very rare cell types
- In clinical settings
- T-cell isolation from patients for cancer therapy
- Higher affinity than antibodies for certain targets (high specificity)
- Modular design / Flexibility
- Control over number of copies of specific affinity agent used on protein scaffold for detection
- Oligomer sequence can be modified for very specific targeting of antigen
- Allows for the sorting of extremely rare cell types
State Of Development
The invention is currently in the testing phase using mouse and human samples. Development is ongoing. U.S. and international patents have been filed.
|United States Of America
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