Redox-Based Reagents For Methionine Bioconjugation

Patent Status

Brief Description

This invention relates to Redox-Based Reagents for Methionine Bioconjugation, which achieve chemoselective conjugation through redox reactivity. The process involves reacting an N-transfer oxidant with a thioether substrate in an aqueous environment to form a conjugation product. Specifically, Redox-Activated Chemical Tagging (ReACT) strategies are employed for methionine-based protein functionalization. Oxaziridine (Ox) compounds serve as oxidant-mediated reagents for direct functionalization by converting methionine to the corresponding sulfimide conjugation product.

Suggested uses

●      Protein Functionalization: Enhancing or modifying protein functions for research and therapeutic applications.

●      Bioconjugation: Creating conjugates for drug delivery, imaging, or diagnostic purposes.

●      Biomarker Detection: Developing sensitive assays for detecting methionine-containing proteins.

●      Synthetic Biology: Engineering proteins with novel functionalities for various biotechnological applications.

●      Chemical Biology: Studying protein interactions and functions through targeted modifications.

Advantages

●      Chemoselectivity: Achieves specific conjugation at methionine residues without affecting other amino acids.

●      Efficiency: Redox reactivity in aqueous environments ensures rapid and effective conjugation.

●      Versatility: Applicable to a wide range of proteins and peptides.

●      Precision: Oxaziridine compounds enable direct and controlled functionalization.

●      Biocompatibility: Suitable for use in biological systems without adverse effects.

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