Method For Detecting Protein-Specific Glycosylation

Patent Status

Brief Description

O-GlcNAc modification is a common form of post-translational modification that mediates cellular activity and stem cell programming by modifying transcription factors. Multiple human diseases, including cancer and diabetes, have been linked to aberrant O-GlcNAcylation of specific proteins.

Despite the importance of this modification, current methods for detection require advanced instrumentation and expertise as well as arduously enriched or purified samples. The “Glyco-seq” method developed by UC Berkeley researchers is highly sensitive, easy to use, and enables O-GlcNAc detection on proteins of interest in cell lysate. 

Suggested uses

- High throughput screening
- Clinical diagnostics
- Analytical tool for basic research

Advantages

- No need for sample enrichment or protein purification
- Orders of magnitude more sensitive than current detection methods
- Able to differentiate signal contributions from protein amount and O-GlcNAc amount
- Potential for multiplexing

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