Plant growth and development depends on the coordination of gene expression in a tissue-, temporal-, or signal-dependent manner. Often, the complex expression pattern observed for a given gene derives from regulation at both the transcriptional and post-transcriptional levels. This multi-layered approach to gene control is part of what makes plants robust, adaptable, and efficient as living photosynthetic systems. Complex gene regulation requires a multi-level approach. Current technology for transgene regulation in plants is based almost exclusively on transcriptional activation. For example, tissue-specific and stress-responsive promoters are extensively employed for inducible gene regulation, though many of these conditional promoters are species-specific. Alternative splicing of mRNA is an important mechanism of gene regulation in plants. The two possible consequences of alternative splicing of an mRNA are: (i) to change the protein coding sequence, by inserting or deleting sequences, or by shifting the reading frame, or (ii) to change the fate of the mRNA, by inserting or deleting sequences that target the RNA for degradation, localization, or other processes. This research has identified how to use RNA elements that regulate alternative splicing of mRNAs for inducible control of gene expression.