This invention enables the direct measurement of the comprehensive activity of multiple kinase enzymes simultaneously, thereby enabling the mapping of functional kinase networks.
Current methods to assess the functional state of kinase networks, detect the phosphorylated state of kinases, but do not measure the catalytic activity of kinases. Furthermore, these assays are limited to detecting the inert quantities of proteins which does not accurately reflect changes in functionality of kinase enzymes. Thus, the inventors sought to develop an authentic kinase activity mapping assay that could provide additional information about otherwise undetectable alterations in upstream and downstream phosphorylation circuits. Their assay is relevant to all areas of biology and medicine where kinases or phosphorylation events are implicated, from oncology to cardio-vascular diseases, neurodegenerative disorders, or basic biochemistry and cell biology studies.
This novel invention provides the following advantages:
University of California San Francisco scientists, at the forefront of cancer biology, have developed a novel platform to enable the direct measurement of kinase activity within a cell.
To develop and commercialize this technology as a research tool.
Professor Laura Van'T Veer: http://profiles.ucsf.edu/laura.vantveer