NOVEL, HIGH THROUGHPUT METHOD TO MEASURE THE FUNCTIONAL ACTIVITY OF MULTIPLE KINASE ENZYMES SIMULTANEOUSLY

Tech ID: 24878 / UC Case 2014-125-0

Invention Novelty

This invention enables the direct measurement of the comprehensive activity of multiple kinase enzymes simultaneously, thereby enabling the mapping of functional kinase networks.   

Value Proposition

Current methods to assess the functional state of kinase networks, detect the phosphorylated state of kinases, but do not measure the catalytic activity of kinases.  Furthermore, these assays are limited to detecting the inert quantities of proteins which does not accurately reflect changes in functionality of kinase enzymes.  Thus, the inventors sought to develop an authentic kinase activity mapping assay that could provide additional information about otherwise undetectable alterations in upstream and downstream phosphorylation circuits.  Their assay is relevant to all areas of biology and medicine where kinases or phosphorylation events are implicated, from oncology to cardio-vascular diseases, neurodegenerative disorders, or basic biochemistry and cell biology studies.

 This novel invention provides the following advantages:

 

  •  Directly and specifically measures activity of multiple kinase enzymes simultaneously
  • Evaluates all-inclusive phospho-sensor peptide targets for each kinase
  • Robust, quantifiable high throughput system
  • Yields phospho-signature of both healthy and diseased cells
  • Enables mapping of functional kinase networks
  • Enhances screening of kinase inhibitors 
  • Operationally simple approach

 

Technology Description

University of California San Francisco scientists, at the forefront of cancer biology, have developed a novel platform to enable the direct measurement of kinase activity within a cell.  

Application

  • Pharmacological and small compound screens
  • Laboratory research and discovery

Looking for Partners

To develop and commercialize this technology as a research tool.  

Stage of Development

Preclinical

Data Availability

Under NDA/CDA

INVENTORS PROFILE

Professor Laura Van'T Veer:  http://profiles.ucsf.edu/laura.vantveer

 

Patent Status

Patent Pending

Contact

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Other Information

Keywords

Functional Kinase Networks, Kinase Signatures

Categorized As