Engineering Cas12a Genome Editors with Minimized Trans-Activity

Tech ID: 32640 / UC Case 2022-071-0

Patent Status

Country Type Number Dated Case
Patent Cooperation Treaty Published Application WO 2023/147240 08/03/2023 2022-071

Additional Patent Pending

Brief Description

The inventors engineered a set of LbCas12a mutants through rational design and directed evolution. The engineered mutants can function as efficient genome editors with minimized trans-activity.

Suggested uses

Suggested uses for the engineered LbCas12a mutants with minimized trans-activity include:

  • reduced off-target genome editing in eukaryote cells. 
  • development into other molecular tools for genome editing.


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  • Doudna, Jennifer A.

Other Information


genome editing

Categorized As

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