Modifications To Cas9 For Passive-Delivery Into Cells
Patent Status
| Country | Type | Number | Dated | Case |
| United States Of America | Issued Patent | 11,118,194 | 09/14/2021 | 2016-016 |
Brief Description
RNA-mediated adaptive immune systems in bacteria and archaea
rely on Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)
genomic loci and CRISPR-associated (Cas) proteins that function together to
provide protection from invading viruses and plasmids. In Type II CRISPR-Cas
systems, the Cas9 protein functions as an RNA-guided endonuclease that uses a
dual-guide RNA consisting of crRNA and trans-activating crRNA (tracrRNA) for
target recognition and cleavage by a mechanism involving two nuclease active
sites that together generate double-stranded DNA breaks (DSBs). Thus, the Cas9
system provides a facile means of modifying genomic information.
UC Berkeley researchers have developed modified
site-directed modifying polypeptides and ribonucleoproteins comprising the
modified polypeptides. As the modified site-directed modifying polypeptides are
modified for passive entry into target cells, the polypeptides are useful in a
variety of methods for target nucleic acid modification.
Suggested uses
- Genome editing
- Gene therapy
Advantages
- Crosses the plasma membrane of a eukaryotic cell without the need for any additional agent (e.g., small molecule agents, lipids, etc.)
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Doudna, Jennifer A.
Other Information
Keywords
CRISPR, Cas9, gene, genome editing
Additional Technologies by these Inventors
- COMPOSITIONS AND METHODS FOR IDENTIFYING HOST CELL TARGET PROTEINS FOR TREATING RNA VIRUS INFECTIONS
- Genome Editing via LNP-Based Delivery of Efficient and Stable CRISPR-Cas Editors
- Type III CRISPR-Cas System for Robust RNA Knockdown and Imaging in Eukaryotes
- Cas12-mediated DNA Detection Reporter Molecules
- Improved guide RNA and Protein Design for CasX-based Gene Editing Platform
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- RNA-directed Cleavage and Modification of DNA using CasY (CRISPR-CasY)
- CasX Nickase Designs, Tans Cleavage Designs & Structure
- In Vivo Gene Editing Of Tau Locus Via Liponanoparticle Delivery
- A Dual-RNA Guided CasZ Gene Editing Technology
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF (“Cas-VariPhi”)
- A Protein Inhibitor Of Cas9
- RNA-directed Cleavage and Modification of DNA using CasX (CRISPR-CasX)
- Compositions and Methods for Genome Editing
- Split-Cas9 For Regulatable Genome Engineering
- NANOPORE MEMBRANE DEVICE AND METHODS OF USE THEREOF
- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
- CRISPR CASY COMPOSITIONS AND METHODS OF USE
- Single Conjugative Vector for Genome Editing by RNA-guided Transposition
- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
- Engineered/Variant Hyperactive CRISPR CasPhi Enzymes And Methods Of Use Thereof
- Engineering Cas12a Genome Editors with Minimized Trans-Activity
- Methods Of Use Of Cas12L/CasLambda In Plants
- Type V CRISPR/CAS Effector Proteins for Cleaving ssDNA and Detecting Target DNA
- THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Endoribonucleases For Rna Detection And Analysis
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- CRISPR-Cas Effector Polypeptides and Methods of Use Thereof
- Class 2 CRISPR/Cas COMPOSITIONS AND METHODS OF USE
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Identification Of Sites For Internal Insertions Into Cas9
- Methods and Compositions for Controlling Gene Expression by RNA Processing
