TRM:Sox9CreER BAC Transgenic Mice

Background

These transgenic mice express an inducible version of cre recombinase mice under the direction of a Sox9 promoter. They are suitable for performing cre-recombination in pancreatic ductal cells and their progenitors.

Technology Description

Mice hemizygous for the BAC Sox9-creER^T2 transgene are viable and fertile. Cre-ER^T2 expression is directed by the murine SRY-box containing gene 9 (Sox9) promoter/enhancer regions in early pancreatic progenitor and ductal cells. Restricted to the cytoplasm, Cre-ER^T2 can only gain access to the nuclear compartment after exposure to tamoxifen. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring. 

Applications

Applications include the following:

• Research Tools

• Genetics Research

• Tissue/Cell Markers: pancreatic beta cells

• Cre-lox System

• Cre Recombinase Expression: Inducible

State Of Development

 The mice are designated Tangible Research Material (TRM).  A complete description, including genotyping, phenotyping, etc is found at The Jackson Lab cat. No. 018829 https://www.jax.org/strain/018829

Intellectual Property Info

Academic and non-profit institutions please order directly from The Jackson Laboratory. Commercial entities require a license from UC San Diego contact ( https://innovation.ucsd.edu/contact/).

Related Materials

• Kopp JL, Dubois CL, Schaffer AE, Hao E, Shih HP, Seymour PA, Ma J, Sander M. Sox9+ ductal cells are multipotent progenitors throughout development but do not produce new endocrine cells in the normal or injured adult pancreas. Development. 2011 Feb;138(4):653-65. doi: 10.1242/dev.056499. - 02/15/2011