A major goal in disease screening, diagnosis, and control has been to develop bioassay platforms capable of simultaneous measurements of different analytes in a single assay. Significant advances toward multiplexed biomarker detection chips based on either immunoassays or enzymatic bioassays have thus been reported. However, the combination of enzymatic and immunoassay sensing into a single disposable system has hitherto not been addressed.
Researchers at UC San Diego have developed a disposable electrochemical chip platform for on-the-spot monitoring of multiple diabetes-relevant biomarkers found in human blood, saliva and interstitial fluid. In an exemplary embodiment, a dual‐marker biosensor integrates enzyme and antibody‐based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing was realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase‐labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30 min, including direct measurements in whole blood and saliva samples.
Personalized, point‐of‐care multiplexed biomarker detection
Inexpensive, wearable integrated enzymatic‐immunoassay biosensor
Provisional patent application
Biosensors, diabetes monitoring, dual-marker detection, electrochemistry, immunoassays