Site-Specific Coupling Of Biomolecules Using Orthoquinones And Thiols

Tech ID: 30184 / UC Case 2019-106-0

Patent Status

Country Type Number Dated Case
Australia Published Application WO 2020/197934 10/01/2020 2019-106
Canada Published Application WO 2020/197934 10/01/2020 2019-106
China Published Application WO 2020/197934 10/01/2020 2019-106
European Patent Office Published Application WO 2020/197934 10/01/2020 2019-106
India Published Application WO 2020/197934 10/01/2020 2019-106
Japan Published Application WO 2020/197934 10/01/2020 2019-106
Rep Of Korea Published Application WO 2020/197934 10/01/2020 2019-106
Patent Cooperation Treaty Published Application WO2020197934 10/01/2020 2019-106
 

Additional Patent Pending

Brief Description

The inventors have developed an enzymatically catalyzed method for simple and rapid coupling of biomolecules to native amino acids on protein surfaces. This method is capable of attaching tyrosine or phenol containing molecules, peptides, or proteins to cystine or thiol containing targets at neutral pH with high yields. The inventors demonstrate the utility of this system by modifying Cas9 and other proteins with fluorophores, peptides, and whole proteins, such as green fluorescent proteins (GFPs) and antibody short chain variable fragments. This technology represents a novel paradigm in biomolecule coupling.

Suggested uses

This technology has significant implications in delivery of CRISPR proteins as therapeutics, antibody conjugation for immune based therapies, biomaterial construction, and vaccine development.

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Inventors

  • Francis, Matthew B.

Other Information

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