Methods for Measuring Protein-DNA Interactions with Long-read DNA Sequencing
Tech ID: 32321 / UC Case 2021-127-0
Patent Status
| Country | Type | Number | Dated | Case |
| Patent Cooperation Treaty | Published Application | WO 2022/256469 | 12/08/2022 | 2021-127 |
Additional Patent Pending
Brief Description
The inventors present a method to modify DNA at the site of protein-DNA interactions in intact nuclei that takes advantage of the ability for long-read DNA sequencing platforms to detect base pair modifications, such as methylation, to map the location of interactions.
Current techniques for profiling protein-DNA interactions with sequencing are limited by short-read DNA sequencing technology, which precludes the mapping of protein-DNA interactions in highly repetitive regions of the genome.
Suggested uses
Suggested uses for this technology include:
- as a research tool in academic and commercial labs to map protein-DNA interactions human cells and other organisms.
- identifying regulatory mechanisms associated with protein binding in the genome that may lead to therapeutic insight. For example, investigators could elucidate the mechanisms of genetic diseases by coupling this invention with functional genomic screens of genetic variants associated with chromatin organization.
Advantages
The new method presented by the inventors maps protein-DNA interactions genome-wide, including highly repetitive areas of the genome.
In contrast, other high-throughput DNA sequencing platforms can only sequence short snippets of DNA molecules, typically around 200 base pairs in length.
Related Materials
Contact
- Terri Sale
- terri.sale@berkeley.edu
- tel: View Phone Number.
Inventors
- Streets, Aaron Michael
Other Information
Keywords
chromatin organization, protein-DNA interactions, epigenomics, genomics, DNA sequencing
