Site-Specific Ligation and Compound Conjugation to Existing Antibodies

Tech ID: 27202 / UC Case 2015-138-0

Abstract

Researchers at the University of California, Davis have developed a unique method for site-specific ligation and conjugation of compounds to existing antibodies.

Full Description

Traditionally, non-specific ligation methods have been used to chemically modify immunoglobulins. Through these methods, cytotoxic compounds are generally conjugated to antibodies via targeting of: (i) amines of lysine residues and (ii) free sulfhydryls of cysteine residues. These modifications, however, lead to heterogeneous products due to the multiple instances of identical functional groups. Therefore, there is a need for site-specific modification of immunoglobulins to establish homogenous immunoconjugates.

Researchers at the University of California, Davis have developed a unique method for site-specific ligation and conjugation of compounds to antibodies via direct binding of a ligand to the Fab arm. This method can be used to conjugate compounds to existing monoclonal and polyclonal antibodies, reducing production costs and shortening the preclinical-to-clinical translation times. The modification itself can be performed in very mild conditions, providing a viable method to create uniform antibody drug conjugates and bispecific antibodies. The method has been verified in vitro with human breast cancer cells and trastuzmab.

Applications

  • Therapeutics against cancer or infectious agents
  • Bispecific antibodies

Features/Benefits

  • Reduced production costs
  • Reduced preclinical-to-clinical translation times
  • Mild reaction conditions
  • Site-specific ligation and conjugation
  • Can be used with existing antibodies

Related Materials

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180140715 05/24/2018 2015-138
 

Additional Patent Pending

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Inventors

  • Bharadwaj, Gaurav
  • Chandrasekaran, Siddarth
  • Feng, Chun
  • Lac, Diana
  • Lam, Kit S.

Other Information

Keywords

anti-body drug conjugates, site specific linker, fab crosslinker, chemotherapy

Categorized As