Homoallylamines As Formaldehyde-Responsive Triggers With Imaging Applications

Tech ID: 25312 / UC Case 2016-024-0

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180215773 08/02/2018 2016-024
 

Brief Description

The invention concerns the use of homoallylamines which undergo a 2-aza-Cope reaction upon condensation with formaldehyde (FA) which can be coupled to a ftuorogenic, colorimetric, or biolurninescent response. Traditional methods for biological FA detection rely on sample destruction and/or extensive processing, resulting in a loss of spatiotemporal information. The invention, as showcased by the tw'! chemical probes FAP-1 and FP-1, enables detection of biological FA with high selectivity in aqueous buffer and in living samples in a non-invasive manner. The hornoallylamine trigger can be generalized to cage a large variety of different fluorophores, chromophores, and bioluminescent molecules (e.g. tuciferin). 

Suggested uses

Measuring FA in aqueous buffer (e.g. as a proxy for demethylase enzyme activity, potentially in a high-throughput screen format), for fluorescent imaging of FA in cell culture, and for bioluminescent imaging of FA in live animals. 

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Inventors

  • Chang, Christopher J.

Other Information

Keywords

Fluorescence, sensor, probe, formaldehyde, demethylase enzyme

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