|United States Of America||Issued Patent||10,124,312||11/13/2018||2014-176|
The ability to attach living cells to surfaces has enabled the study of many key behaviors in biology, including immune synapse formation, stem cell differentiation, cancer cell motility, and drug response. However, most of the previous surface patterning methods require interactions between the surface integrins of adherent mammalian cells with proteins bearing "RGD" motifs.
Scientists at UC Berkeley have developed an alternative method of surface patterning in which synthetic DNA strands introduced on the cell surfaces bind to sequence complements displayed on the binding surface. Demonstrated advantages of this method include its generality for all biological cell types, exceptionally high precision and efficiency, and ability to generate complex multicellular patterns through the use of multiple capture sequences.
drug screening, drug response, surface patterning, diagnostics, cell motility, synthetic biology