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Symmetric Redox Flow Batteries for Economically-Viable Grid-Scale Energy Storage

A 5-redox state nitride-capped organometallic motif that can completely replace current redox flow batteries.

Method For Indefinite Storage And Preservation Of Membrane Precursors

UCLA researchers in the Department of Bioengineering have developed a novel strategy for the creation of biomimetic lipid bilayer membrane using a high freezing point lipid-containing solvent.  Using this method, the membrane precursor is frozen/immobilized prior to the completion of the spontaneous process of bilayer self-assembly, and the process can be resumed later by simply thawing and allowing membrane formation to resume.

Engineering Polyketide Synthase Machinery in Cyanobacteria

Complex polyketides include a family of natural products that possess a wide variety of pharmacological or biological activities. Numerous polyketides and their semisynthetic derivatives have been approved for clinical use in humans or animals, including antibiotics, antifungal agents, immunosuppressants, antiparasitic agents and insecticides. All these natural products share a common mechanism of biosynthesis and are produced by a class of enzymes called polyketide synthases (PKSs). Besides their essential role in the biosynthesis of a vast diversity of natural products, the versatility of PKSs can be further emphasized as they can be redesigned and repurposed to produce novel molecules that could be used as fuels, industrial chemicals, and monomers. Most polyketide producers are slow-growing, recalcitrant to genetic manipulation, or even non-culturable.

1,4-Butanediol Production In Microorganisms

UCLA researchers in the Department of Chemical and Biomolecular Engineering have constructed a novel metabolic system for production of 1,4-butanediol from renewable carbohydrate feedstocks using microorganisms such as Escherichia coli.

Production of C7 Alcohol (2-Isopropyl-1-Butanol) in Escherichia Coli by Combining Protein Evolution and Metabolic Engineering

UCLA researchers in the Department of Chemical and Biomolecular Engineering have developed metabolically-modified microorganisms for producing the biofuel 2-isopropyl-1-butanol.

Synthetic Algal Promoters as a Tool for Increasing Nuclear Gene Expression in Green Algae

Algae have enormous potential as bio-factories for the efficient production of a wide array of high-value products, and eventually as a source of renewable biofuels. However, tools for engineering the nuclear genomes of algae remain scarce and limited in functionality, in part due to lack of strong promoters.

An Anaerobic Photo-Fermentation Processes For Production Of Volatile Non-Methane Hydrocarbons

An anaerobic process to produce non-methane hydrocarbon gases from organic and inorganic substrates.

A Highly Error-Prone Orthogonal Replication System For Targeted Continuous Evolution In Vivo

Inventors at UC Irvine have engineered an orthogonal DNA replication system capable of rapid, accelerated continuous evolution. This system enables the directed evolution of specific biomolecules towards user-defined functions and is applicable to problems of protein, enzyme, and metabolic pathway engineering.

Non-Oxidative Glycolysis For Production Of Acetyl-CoA Derived Compounds

The Liao group at UCLA has constructed a Non-Oxidative Glycolysis pathway for the synthesis of biofuel precursors with a 100% carbon conversion rate.

Hydrocarbon Production, H2 Evolution And CO2 Conversion By Whole Cells Or Engineered Azotobacter Vinelandii Strains

Using metal catalysts in industrial synthesis of hydrocarbons for fuels can be costly, inefficient, and harmful to the environment. This simple approach uses genetically-modified soil bacterium to synthesize valuable hydrocarbons using recycled components. This novel process is environmentally-friendly and is more cost- and energy-efficient than current industrial synthesis.

Biomass-Derived Polymers And Copolymers Incorporating Monolignols And Their Derivatives

UCLA researchers in the Departments of Bioengineering, Chemistry and Biochemistry have developed a novel synthetic strategy for the fabrication of biomass-derived polymers incorporating underutilized lignin derivatives.

Rapid, Portable And Cost-Effective Yeast Cell Viability And Concentration Analysis Using Lensfree On-Chip Microscopy And Machine Learning

UCLA researchers in the Department of Electrical Engineering have developed a new portable device to rapidly measure yeast cell viability and concentration using a lab-on-chip design.

Organic Waste Material Treatment

A researcher at the University of California, Davis has developed a method for treating organic waste materials.

Renewable Energy Synthesis System

Researchers at the University of California, Davis have developed a novel system for acetoin and 2,3-butanediol synthesis from carbon dioxide.

Method and System for Ultra High Dynamic Range Nucleic Acid Quantification

Researchers at UC Irvine developed a device and method that combines the high dynamic range and high accuracy of digital PCR (dPCR) with the real-time analysis of quantitative PCR (qPCR) to achieve a ultra-high dynamic range PCR over 10 to 12 orders of magnitude. The present method is accomplished by a highly integrated design that optimally packs, thermocycles, and images as many as 1 million reaction vessels.

Self-Adaptive Control And Optimization Of Ultrafiltration

UCLA researchers in the Department of Chemical and Biomolecular engineering have developed a novel UF-RO system.

Clarifying Water And Wastewater With Fungal Treatment/Bioflocculation

Researchers at the University of California, Davis have developed a low cost method of cleaning water and wastewater by removing microalgae and bacteria with fungal bioflocculation.

Mammalian Cell Culture Optimization

Biotherapeutic proteins manufactured in cell culture systems have transformed modern medicine. Selling many tens of billions per year, new biotherapeutics such as monoclonal antibodies have delivered dramatic clinical results, while posing significant manufacturing problems.: During the cell culture manufacturing process, toxic bioproducts such as lactate and ammonia have posed considerable challenges in bioprocessing, since they limit cell growth and impact critical quality attributes of recombinant protein production (e.g., therapeutic drugs, enzymes). That is because the lactate alters the regulation of biosynthetic enzymes, and can lead to changes in pH in the culture. To mitigate the negative effects of lactic acid accumulation and control the culture pH, chemical ‘base’ is added to the media during the course of a bioprocess. However, the base addition negatively impacts the bioprocess by inhibiting growth and shortening the length of time in which the cells can produce the recombinant protein. This leads to reduced yield, and increased cost-of-goods. Thus, it is of great interest to eliminate lactate production, and UC San Diego researchers have recently developed a new process for achieving this.  

Production of Glycolipid PEFAs from Yeasts

Method of using basidiomycetous yeasts to convert carbohydrates to glycolipid biosurfactants

Controllable Emulsification And Point-Of-Care Assays Driven By Magnetic Induced Movement Of The Fluid

UCLA researchers in the department of Bioengineering have developed a novel microfluidic droplet generation technique, where instead of pumps, only magnetic force is used for controllable emulsification of ferrofluid containing solutions. 

Enzymatic Synthesis Of Cyclic Dinucleotides

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} GGDEF domain-containing enzymes are diguanylate cyclases that produce cyclic di-GMP (cdiG), a second messenger that modulates the key bacterial lifestyle transition from a motile to sessile biofilm-forming state. The ubiquity of genes encoding GGDEF proteins in bacterial genomes has established the dominance of cdiG signaling in bacteria. A subfamily of GGDEF enzymes synthesizes the asymmetric signaling molecule cyclic AMP-GMP. Hybrid CDN-producing and promiscuous substrate-binding (Hypr) GGDEF enzymes are widely distributed and found in other deltaproteobacteria and have roles that include regulation of cAG signaling.  GGDEF enzymes that produce cyclic dinucleotides are especially of interest.    UC Berkeley researcher have developed a new method of preparing and using cyclic dinucleotides (CDNs) by contacting a CDN producing-enzyme (e.g., a GGDEF enzyme) with a precursor of a CDN under conditions sufficient to convert the precursor into a CDN. This method produces a variety of non-naturally occurring, asymmetric and symmetric CDNs and can be performed in vitro or in a genetically modified host cell. Also provided are CDN compositions that find use in a variety of applications such as modulating an immune response in an individual.  

A Low-Profile Flow Shear Sensing Unit

UCLA researchers have developed an accurate low-profile shear sensing unit that is viable for both gas and liquid flows.

Nanoscale Optical Voltage Sensors

UCLA researchers have developed a novel nanoscale optical voltage sensor.

Novel Synthesis of 2,5- Dimethylfuran from 5- (Chloromethyl)furfural

Researchers at the University of California, Davis have developed an efficient synthesis of 2,5- dimethylfuran (DMF) from 5- (chloromethyl)furfural (CMF).

Salmonella-Based Gene Delivery Vectors and their Preparation

Nucleic acid-based gene interference technologies, including ribozymes and small interfering RNAs (siRNAs), represent promising gene-targeting strategies for specific inhibition of mRNA sequences of choice. A fundamental challenge to use nucleic acid-based gene interfering approaches for gene therapy is to deliver the gene interfering agents to appropriate cells in a way that is tissue/cell specific, efficient and safe. Many of the currently used vectors are based on attenuated or modified viruses, or synthetic vectors in which complexes of DNA, proteins, and/or lipids are formed in particles, and tissue-specific vectors have been only partially obtained by using carriers that specifically target certain cell types. As such, efficient and targeted delivery of M1GS sequences to specific cell types and tissues in vivo is central to developing this technology for gene targeting applications. Invasive bacteria, such as Salmonella, possess the ability to enter and transfer genetic material to human cells, leading to the efficient expression of transferred genes. Attenuated Salmonella strains have earlier been shown to function as a carrier system for delivery of nucleic acid-based vaccines and anti-tumor transgenes. Salmonella-based vectors are low cost and easy to prepare. Furthermore, they can be administrated orally in vivo, a non-invasive delivery route with significant advantage. Thus, Salmonella may represent a promising gene delivery agent for gene therapy. Scientists at UC Berkeley have developed a novel attenuated strain of Salmonella, SL101, which exhibited high gene transfer activity and low cytotoxicity/pathogenicity while efficiently delivering ribozymes, for expression in animals. Using MCMV infection of mice as the model, they demonstrated that oral inoculation of SL101 in animals efficiently delivered RNase P-based ribozyme sequence into specific organs, leading to substantial expression of ribozyme and effective inhibition of viral infection and pathogenesis. This strategy could easily be adopted deliver other gene targeting technologies.

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