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Biomass-Derived Polymers And Copolymers Incorporating Monolignols And Their Derivatives

UCLA researchers in the Departments of Bioengineering, Chemistry and Biochemistry have developed a novel synthetic strategy for the fabrication of biomass-derived polymers incorporating underutilized lignin derivatives.

A Hybrid Pathway for the In Vitro Conversion of Glucose to Acetyl-CoA Derived Products

UCLA researchers in the Department of Chemistry and Biochemistry have developed an in vitro system for production of bioplastics.

A Molecular Rheostat Design that Maintains ATP Levels Needed to Drive Cell-Free Synthetic Biochemistry Systems

UCLA researchers in the Department of Chemistry and Biochemistry have developed a system for regulation of cofactors in cell free biochemical production.

High-Throughput And Label-Free Single Nanoparticle Sizing Based On Time-Resolved On-Chip Microscopy

UCLA researchers in the Department of Electrical Engineering have developed a rapid, low-cost, and label-free methodology for nanoparticle sizing.

Rapid, Portable And Cost-Effective Yeast Cell Viability And Concentration Analysis Using Lensfree On-Chip Microscopy And Machine Learning

UCLA researchers in the Department of Electrical Engineering have developed a new portable device to rapidly measure yeast cell viability and concentration using a lab-on-chip design.

Organic Waste Material Treatment

A researcher at the University of California, Davis has developed a method for treating organic waste materials.

Renewable Energy Synthesis System

Researchers at the University of California, Davis have developed a novel system for acetoin and 2,3-butanediol synthesis from carbon dioxide.

Method and System for Ultra High Dynamic Range Nucleic Acid Quantification

Researchers at UC Irvine developed a device and method that combines the high dynamic range and high accuracy of digital PCR (dPCR) with the real-time analysis of quantitative PCR (qPCR) to achieve a ultra-high dynamic range PCR over 10 to 12 orders of magnitude. The present method is accomplished by a highly integrated design that optimally packs, thermocycles, and images as many as 1 million reaction vessels.

Self-Adaptive Control And Optimization Of Ultrafiltration

UCLA researchers in the Department of Chemical and Biomolecular engineering have developed a novel UF-RO system.

Clarifying Water And Wastewater With Fungal Treatment/Bioflocculation

Researchers at the University of California, Davis have developed a low cost method of cleaning water and wastewater by removing microalgae and bacteria with fungal bioflocculation.

Mammalian Cell Culture Optimization

Biotherapeutic proteins manufactured in cell culture systems have transformed modern medicine. Selling many tens of billions per year, new biotherapeutics such as monoclonal antibodies have delivered dramatic clinical results, while posing significant manufacturing problems.: During the cell culture manufacturing process, toxic bioproducts such as lactate and ammonia have posed considerable challenges in bioprocessing, since they limit cell growth and impact critical quality attributes of recombinant protein production (e.g., therapeutic drugs, enzymes). That is because the lactate alters the regulation of biosynthetic enzymes, and can lead to changes in pH in the culture. To mitigate the negative effects of lactic acid accumulation and control the culture pH, chemical ‘base’ is added to the media during the course of a bioprocess. However, the base addition negatively impacts the bioprocess by inhibiting growth and shortening the length of time in which the cells can produce the recombinant protein. This leads to reduced yield, and increased cost-of-goods. Thus, it is of great interest to eliminate lactate production, and UC San Diego researchers have recently developed a new process for achieving this.  

Environmentally friendly whitening of cotton fabrics

Researchers at the University of California, Davis have developed a method of whitening raw cotton fabrics or citric acid treated cotton fabrics by bleaching with hydrogen peroxide (H2O2) at neutral conditions under ultraviolet (UV) exposure.

Production of Glycolipid PEFAs from Yeasts

Method of using basidiomycetous yeasts to convert carbohydrates to glycolipid biosurfactants

Controllable Emulsification And Point-Of-Care Assays Driven By Magnetic Induced Movement Of The Fluid

UCLA researchers in the department of Bioengineering have developed a novel microfluidic droplet generation technique, where instead of pumps, only magnetic force is used for controllable emulsification of ferrofluid containing solutions. 

Enzymatic Synthesis Of Cyclic Dinucleotides

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} GGDEF domain-containing enzymes are diguanylate cyclases that produce cyclic di-GMP (cdiG), a second messenger that modulates the key bacterial lifestyle transition from a motile to sessile biofilm-forming state. The ubiquity of genes encoding GGDEF proteins in bacterial genomes has established the dominance of cdiG signaling in bacteria. A subfamily of GGDEF enzymes synthesizes the asymmetric signaling molecule cyclic AMP-GMP. Hybrid CDN-producing and promiscuous substrate-binding (Hypr) GGDEF enzymes are widely distributed and found in other deltaproteobacteria and have roles that include regulation of cAG signaling.  GGDEF enzymes that produce cyclic dinucleotides are especially of interest.    UC Berkeley researcher have developed a new method of preparing and using cyclic dinucleotides (CDNs) by contacting a CDN producing-enzyme (e.g., a GGDEF enzyme) with a precursor of a CDN under conditions sufficient to convert the precursor into a CDN. This method produces a variety of non-naturally occurring, asymmetric and symmetric CDNs and can be performed in vitro or in a genetically modified host cell. Also provided are CDN compositions that find use in a variety of applications such as modulating an immune response in an individual.  

A Low-Profile Flow Shear Sensing Unit

UCLA researchers have developed an accurate low-profile shear sensing unit that is viable for both gas and liquid flows.

Nanoscale Optical Voltage Sensors

UCLA researchers have developed a novel nanoscale optical voltage sensor.

ENGINEERED MICROORGANISMS FOR PRODUCTION OF COMMODITY CHEMICALS AND CELLULAR BIOMASS

96 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Citrus pulp and sugar beet pulp are pectin-rich agricultural wastes that are globally produced in significant amounts and have the potential to contribute towards the greater bioeconomy as a source of raw, inexpensive carbohydrate biomass. There is currently limited use for these waste streams. In some cases, pulps are dried, pelleted, and repurposed as an inexpensive livestock feed, however this application is barely profitable due to high production costs. There is a need for technologies that can cost-effectively transform pectin-rich waste streams into value-added products of commercial interest.   UC Berkeley researchers developed an efficient microbial strain technology and metabolic fermentation methods for the bioconversion of pectin-rich waste streams to useful bio-based commodity chemicals and biofuels. In addition to the beneficial environmental impact of utilizing a waste-stream, the fermentation technologies achieve three design goals set to optimize the productivity of bioconversions and economic viability. First, the technology allows for anaerobic fermentation, eliminating the need for culture oxygenation. This lowers operating costs by simplifying the metabolic requirements of high-density fermentation cultures. Second, co- utilization of the major component monosaccharides in the hydrolysate broth allows for productive conversion of the predominant, energy- rich biomass sugars. Third, fermentations can be conducted at low pH, discouraging contaminant growth and eliminating the need to buffer the hydrolysate mixture.  

Novel Synthesis of 2,5- Dimethylfuran from 5- (Chloromethyl)furfural

Researchers at the University of California, Davis have developed an efficient synthesis of 2,5- dimethylfuran (DMF) from 5- (chloromethyl)furfural (CMF).

Salmonella-Based Gene Delivery Vectors and their Preparation

Nucleic acid-based gene interference technologies, including ribozymes and small interfering RNAs (siRNAs), represent promising gene-targeting strategies for specific inhibition of mRNA sequences of choice. A fundamental challenge to use nucleic acid-based gene interfering approaches for gene therapy is to deliver the gene interfering agents to appropriate cells in a way that is tissue/cell specific, efficient and safe. Many of the currently used vectors are based on attenuated or modified viruses, or synthetic vectors in which complexes of DNA, proteins, and/or lipids are formed in particles, and tissue-specific vectors have been only partially obtained by using carriers that specifically target certain cell types. As such, efficient and targeted delivery of M1GS sequences to specific cell types and tissues in vivo is central to developing this technology for gene targeting applications. Invasive bacteria, such as Salmonella, possess the ability to enter and transfer genetic material to human cells, leading to the efficient expression of transferred genes. Attenuated Salmonella strains have earlier been shown to function as a carrier system for delivery of nucleic acid-based vaccines and anti-tumor transgenes. Salmonella-based vectors are low cost and easy to prepare. Furthermore, they can be administrated orally in vivo, a non-invasive delivery route with significant advantage. Thus, Salmonella may represent a promising gene delivery agent for gene therapy. Scientists at UC Berkeley have developed a novel attenuated strain of Salmonella, SL101, which exhibited high gene transfer activity and low cytotoxicity/pathogenicity while efficiently delivering ribozymes, for expression in animals. Using MCMV infection of mice as the model, they demonstrated that oral inoculation of SL101 in animals efficiently delivered RNase P-based ribozyme sequence into specific organs, leading to substantial expression of ribozyme and effective inhibition of viral infection and pathogenesis. This strategy could easily be adopted deliver other gene targeting technologies.

Redirecting Cytosol Lipid Droplets for Enhanced Production

Background: Lipids (oils) produced by plants and photosynthetic microorganisms are used for general cooking, health food, cosmetics, pharmaceuticals and biodiesel. The current methods to produce oils with photosynthetic microorganisms are inefficient, since the cells must undergo extreme stress for lipid droplet (LD) accumulation and then be killed for extraction. Accumulation of LDs in the cytosol generates metabolic feedback inhibition. Some of these problems also apply to oil production with plants. A more efficient production practice is needed to meet high consumer and commercial demands.  Brief Description: UCR researchers have developed a method to optimize oil synthesis in microorganisms and plants by redirecting cytosolic LDs to the cell vacuoles. They successfully identified and modified a specific protein involved in directing lipids to various areas within the cell. Through restructuring and adding novel peptides, researchers were then able to re-route the fate of lipids into vacuoles (storage warehouses), thus eliminating metabolic feedback inhibition. Currently, they are also working towards achieving redirection of lipids to the cell exterior for excretion.

Fluorescent Biosensor for Methyltransferase Assay

Correct epigenetic regulation is essential to cellular development, and methyltransferases are enzymes important for epigenetic regulatory processes. They add methyl groups to their substrates, which can be DNA, proteins, or small-molecule secondary metabolites. Methyltransferases have been implicated in a number of diseases, including cancer, HIV infection, and diabetes, yet many remain uncharacterized.S-adenosyl methionine (SAM) is used as a methyl group donor by a majority of methyltransferases. Use of SAM by a methyltransferase results in the production of S-adenosyl homocysteine (SAH). SAM is found across all branches of life, and therefore represents a useful biological marker for methyltransferase activity. Researchers at UC Berkeley have developed a sensitive and selective means of assaying methyltransferase activity. This assay monitors the presence of SAH, and can be used for high-throughput screening.

Carbon Sequestration Using a Magnetic Treatment System

The technology is a technique for the capture and removal of carbonates in natural water sources.It features the use of an alternating electromagnetic field (AMF) to induce the formation of calcium carbonate or other carbonate compounds in suspension in water source. Additionally, carbonate compounds are removed using filtration device.

Tunable Vapor-Condensed Nano-Lenses

UCLA researchers in the Department of Electrical Engineering have developed an improved and cost-efficient nanolens to visualize nanoparticles and viral particles with 50 fold greater detection and more than 10 fold field-of-view compared to other imaging modalities.

High-Throughput Rapid Screening Platform For Microalgal Biofuel Applications

Algal photosynthesis is now considered a sustainable alternative and renewable solution for green energy, however, the large number of screening processes required significantly delay the time for the pragmatic applications.  Therefore, the success of algal biofuel energy production depends on the rapidity and efficiency of algal strain selections for various biofuel aspects.   UC Berkeley researchers have developed a high-throughput rapid screening platform for microalgal biofuel applications.  The screening platform enables optical field enhancement with an optical spectrum favorable to photosynthesis and enhanced intercellular interactions.  The platform shows a high rate of population growth and a significant reduction of lag-phase duration.  

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