XNA Aptamer Particle Display Technology

Brief Description

An innovative mid-throughput technique for screening and optimizing threose nucleic acid (TNA) aptamers for protein-binding activity.

Full Description

This technology introduces a novel method for rapidly screening threose nucleic acids (TNA) aptamers for affinity and specificity to a protein target of interest. It leverages bead-based DNA particle display, facilitating the high-throughput selection of aptamers with unique properties such as increased stability and chemical functionality. This advancement extends the application of DNA particle display to XNA-based aptamers, overcoming the previous limitation of DNA polymerase's incompatibility with artificial genetic nucleotides.

Suggested uses

• Drug discovery and development, specifically in the area of therapeutic aptamers 
• Diagnostic tools for detecting specific biomarkers with high affinity and specificity. 
• Research and development in synthetic biology, particularly in the study and application of synthetic genetic materials.

Advantages

• Facilitates mid-throughput screening of unnatural aptamers with desired properties. 
• Compatible with standard fluorescence-activated flow cytometry instruments. 
• Avoids tedious synthesis and purification approaches required for individual aptamer sequences

Patent Status

Additional Patent Pending

Related Materials

• Adriana Lozoya-Colinas, Yutong Yu, and John C. Chaput Journal of the American Chemical Society 2023 145 (47), 25789-25796 DOI: 10.1021/jacs.3c0949