Cross-Linkers to Advance Protein-Protein Interaction Studies

Brief Description

A novel suite of trioxane-based, MS-cleavable cross-linking reagents enhancing protein-protein interaction studies.

Full Description

This technology introduces sulfoxide-containing, MS-cleavable cross-linking reagents with a trioxane cleavable site, designed to improve the study of protein-protein interactions (PPIs). It allows for a more flexible cross-linker design and enhances the capture of PPIs in 3-D space, facilitating the robust identification of cross-linked peptides and providing insights into the architecture of protein complexes without cell engineering.

Suggested uses

• Structural biology research and protein complex architecture elucidation. 
• Drug discovery by targeting specific protein-protein interactions. 
• Biotechnological advancements in understanding cellular functionality and physiological states. 
• Development of novel therapeutic strategies based on the mechanistic functions of protein complexes.

Advantages

• MS-Cleavable Homotrifunctional Cross-Linker, allowing for unique MS-cleavability and unambiguous identification by mass spectrometry. 
• Enables the capture of endogenous protein-protein interactions without cell engineering. 
• Provides distance constraints at specific residues, aiding in the refinement of protein structures. 
• Improves flexibility in cross-linker design with trioxane’s multiple extension sites. 
• Facilitates the identification of cross-linked peptides containing three individual peptides, offering additional structural information. 
• Ensures robust MS-cleavability for simplified identification in multistage tandem mass spectrometry analysis workflows. 
• Trioxane Core Structure enables the development of cross-linkers targeting other residues and allows for bifunctional reactive groups plus an enrichment handle.

Patent Status

Patent Pending