High-Fidelity Cas13a Variants
Tech ID: 33820 / UC Case 2023-968-0
Background
Cas13a can be used as part of the CRISPR-Cas toolkit and it exclusively targets RNA, which makes it a promising tool for RNA detection. As a diagnostic and/or RNA detection tool, Cas13a’s activity is measured by the fluorescence signal of the cleaved reporter RNA, which is quenched before cleaving. However, Cas13a-based technologies exhibit high tolerance for mismatches between the guide-RNA and target RNA of interest, limiting their use for the detection of mutations like single nucleotide polymorphisms (SNPs).
Brief Description
Professor Giulia Palermo and colleagues from the University of California, Riverside and the University of Rochester have developed high-fidelity Cas13a variants with increased sensitivity for base pair mismatches.
The activation of these Cas13a variants can be inhibited with a single mismatch between guide-RNA and target-RNA, a property that can be used for the detection of SNPs associated with diseases or specific genotypic sequences.
Application
- Cas13a variants with increased sensitivity to detect base pair mismatches such as SNPs associated with diseases or specific genotypes.
Patent Status
Patent Pending
Related Materials
Contact
- Grace Yee
- grace.yee@ucr.edu
- tel: View Phone Number.
Other Information
Keywords
CRISPR, Cas13a, SNP, diagnostic
